Document Detail


Resuscitation of Escherichia coli VBNC cells depends on a variety of environmental or chemical stimuli.
MedLine Citation:
PMID:  21447017     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Aims:  To analyse VBNC state induction in Escherichia coli and resuscitation of VBNC suspensions in several conditions. Methods and Results:  VBNC were induced in four media, two temperatures and six strains but only cells produced at 4°C were able to resuscitate. Resuscitation of 14 VBNC suspensions obtained in several conditions occurred in the presence of supernatants of growing cells, in minimal medium supplemented with amino acids or after temperature change, depending on strain. A limited time period beyond no more resuscitation could be observed was also confirmed. Conclusions:  The supernatants positive effect is suggested to be due to a non-proteinaceous molecule and a combination of methionine, glutamine, threonine, serine and asparagine could be used as primary mix for resuscitation experiments. Significance and Impact of the Study:  Escherichia coli resuscitation was already attempted in several conditions but it is the first time that a positive result was observed in minimal medium supplemented with amino acids or after temperature change. The role of amino acids in resuscitation is of special interest since was never reported for any species.
Authors:
Daniela Pinto; Vânia Almeida; Mário Almeida Santos; Lélia Chambel
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-29
Journal Detail:
Title:  Journal of applied microbiology     Volume:  -     ISSN:  1365-2672     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-3-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Journal compilation © 2011 The Society for Applied Microbiology.
Affiliation:
Center for Biodiversity, Functional and Integrative Genomics (BioFIG), Faculty of Sciences, University of Lisbon, Lisbon, Portugal.
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