| Restriction fragments that exert promoter activity during postexponential growth of Bacillus subtilis. | |
| | |
MedLine Citation:
|
PMID: 6309749 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Two restriction fragments of Bacillus subtilis DNA were identified which caused the cat-86 gene present on the promoter cloning plasmid pPL703 to be activated predominantly during postexponential growth of host cells. The postexponential increase was observed in both sporulation-positive strains and in a spoOA mutant of B. subtilis. However, the postexponential increase in the cat-86 gene product, chloramphenicol acetyltransferase, was diminished or not observed when the plasmid-containing cells were grown in the presence of excess glucose. The promoter-containing fragment, designated as 33, was mapped to a site on the B. subtilis chromosome adjacent to hisA. The other fragment, 14, mapped to a site adjacent to ctrA. When present on a high-copy vector, both fragments caused a reduction in the sporulation frequency of host cells. Fragment 33 in high copy number conferred on B. subtilis cells three additional phenotypic changes: brown colony color, intracellular inclusions, and, in a protease-deficient mutant, the production of extracellular protease activity. These activities were observed only in postexponential-phase cultures. |
| | |
Authors:
|
S Mongkolsuk; Y W Chiang; R B Reynolds; P S Lovett |
Related Documents
:
|
23666709 - Age-dependent expression of stress and antimicrobial genes in the hemocytes and siphon ... 11698359 - Analyses of mrp genes during myxococcus xanthus development. 12697329 - The effects of insertional mutations in comq, comp, srfa, spo0h, spo0a and abrb genes o... 1368519 - Comparison of cd composition produced by chimeric cgtases. 21830949 - Barley leaf transcriptome and metabolite analysis reveals new aspects of compatibility ... 11679079 - Sigb, an rna polymerase sigma factor required for osmoprotection and proper differentia... 21448569 - Multiple mechanisms underlie metastasis suppressor function of nm23-h1 in melanoma. 8011699 - Cytokines, growth factors and the loss of negative growth controls in the progression o... 9486539 - Identification of the vertebrate iroquois homeobox gene family with overlapping express... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: Journal of bacteriology Volume: 155 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1983 Sep |
Date Detail:
|
Created Date: 1983-10-21 Completed Date: 1983-10-21 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1399-406 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetyltransferases
/
genetics* Bacillus subtilis / enzymology, genetics*, growth & development Chloramphenicol O-Acetyltransferase Cloning, Molecular DNA Restriction Enzymes Gene Expression Regulation* Operon* Peptide Hydrolases / genetics Plasmids* |
| Chemical | |
Reg. No./Substance:
|
EC 2.3.1.-/Acetyltransferases; EC 2.3.1.28/Chloramphenicol O-Acetyltransferase; EC 3.1.21.-/DNA Restriction Enzymes; EC 3.4.-/Peptide Hydrolases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Characterization of Tn2411 and Tn2410, two transposons derived from R-plasmid R1767 and related to T...
Next Document: Plasmid visualization and nif gene location in nitrogen-fixing Azospirillum strains.