Document Detail

Restriction fragment length polymorphism segregation analysis of the Li locus in Trifolium repens L.
MedLine Citation:
PMID:  1983287     Owner:  NLM     Status:  MEDLINE    
The Li locus in white clover controls the presence of cyanogenic beta-glucosidase (linamarase) activity in leaf tissue, such that plants homozygous for the 'null' allele (li) have no linamarase activity in this tissue. The isolation of a cDNA clone from linamarase mRNA is described. The cDNA clone is used to further characterise alleles of the Li locus. Northern blot analysis shows that plants homozygous for the 'null' allele (li li) produce very reduced levels of mRNA which hybridises to the cDNA. Heterozygous plants (Li li), which have intermediate levels of enzyme activity, produce intermediate levels of mRNA. Southern blot analysis of Hind III digested genomic DNA shows that the white clover genome contains three genes with homology to the linamarase cDNA and that at least two of these genes segregate independently. Analysis of the cosegregation of linamarase activity and the presence of genomic restriction fragments identifies the genomic sequence specifying linamarase structure and indicates either a structural or cis acting control function of the Li locus.
M A Hughes; A L Sharif; M A Dunn; E Oxtoby; A Pancoro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant molecular biology     Volume:  14     ISSN:  0167-4412     ISO Abbreviation:  Plant Mol. Biol.     Publication Date:  1990 Mar 
Date Detail:
Created Date:  1991-09-27     Completed Date:  1991-09-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9106343     Medline TA:  Plant Mol Biol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  407-14     Citation Subset:  IM    
Department of Biochemistry and Genetics, Medical School, Newcastle University, Newcastle upon Tyne, UK.
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MeSH Terms
Blotting, Northern
Blotting, Southern
Cloning, Molecular
Crosses, Genetic
DNA / genetics
Fabaceae / enzymology,  genetics
Plants / enzymology,  genetics*
Plants, Medicinal
Polymorphism, Restriction Fragment Length
RNA, Messenger / genetics
beta-Glucosidase / genetics*
Reg. No./Substance:
0/RNA, Messenger; 9007-49-2/DNA; EC 3.2.1.-/cyanogenic beta-glucosidase; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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