| Restriction fragment length polymorphism (RFLP) analysis provides evidence for a high degree of homology of mitochondrial DNAs from rat hepatomas versus normal rat livers. | |
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MedLine Citation:
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PMID: 1363589 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Where differences have been reported between tumor and normal mitochondrial DNA (mtDNA), they have generally involved limited modifications of the genome (Taira et al., Nucleic Acids Res. 11:1635, 1983; Shay and Werbin, Mutat. Res. 186:149, 1987). However, Corral et al. (Nucleic Acids Res. 16:10935, 1988; 17:5191, 1989) observed recombination between cytochrome oxidase subunit I (COI) and NADH dehydrogenase subunit 6 (ND6), two genes normally on opposite sides of the circular mitochondrial genome. In rat hepatoma mtDNA COI and ND6 were reported to be separated by only 230 base pairs (Corral et al., 1988, 1989). We have performed RFLP analysis on mtDNA from normal rat livers and rat hepatomas, using COI and ND6 probes. Additional experiments compared end-labeled DNA fragments produced by EcoRI and HindIII digestion of mtDNA. These studies failed to provide any evidence for genetic recombination in rat hepatoma mtDNA, even in the same cell line used by Corral et al. Rather, they support the conclusion that mtDNA from tumor and normal tissues exhibits a low degree of heterogeneity. |
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Authors:
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R A Nakashima; X Li; J M Bayouth; W C Wigley |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical genetics Volume: 30 ISSN: 0006-2928 ISO Abbreviation: Biochem. Genet. Publication Date: 1992 Dec |
Date Detail:
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Created Date: 1993-04-22 Completed Date: 1993-04-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0126611 Medline TA: Biochem Genet Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 545-56 Citation Subset: IM |
Affiliation:
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Department of Chemistry and Biochemistry, Texas Tech University, Lubbock 79409. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence DNA Mutational Analysis DNA, Mitochondrial / genetics* DNA, Neoplasm / genetics* Electron Transport Complex IV / genetics Gene Rearrangement Liver / chemistry* Liver Neoplasms, Experimental / chemistry*, genetics Male Molecular Sequence Data NADH Dehydrogenase / genetics Polymorphism, Restriction Fragment Length* Rats Rats, Sprague-Dawley Recombination, Genetic |
| Chemical | |
Reg. No./Substance:
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0/DNA, Mitochondrial; 0/DNA, Neoplasm; EC 1.6.99.3/NADH Dehydrogenase; EC 1.9.3.1/Electron Transport Complex IV |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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