| Restriction enzyme analysis of PCR products. | |
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MedLine Citation:
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PMID: 19768608 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Progress in single nucleotide polymorphism (SNP) detection technologies has provided information for SNP-based studies, such as identification of candidate genes for the complex genetic diseases, pharmacogenetic analysis, drug development, population genetics, evolutionary studies, and forensic investigations. SNP detection is performed by many methods, including hybridization, allele-specific polymerase chain reaction (PCR), primer extension, oligonucleotide ligation, direct DNA sequencing, and endonuclease cleavage. Each of these methods has its specific advantages and disadvantages. Here we introduce the PCR-restriction fragment length polymorphism (RFLP) method, which has certain advantages over many other techniques used for analysis of SNPs. The PCR-RFLP method allows very rapid, simple, and inexpensive detection of point mutations within the sequences of PCR products. The mutation is discriminated by the specific restriction endonuclease and is identified by gel electrophoresis followed by staining with ethidium bromide. This convenient and simple method is useful in a small basic research study. |
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Authors:
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Masao Ota; Hideki Asamura; Takahito Oki; Masaharu Sada |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 578 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2009 |
Date Detail:
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Created Date: 2009-09-21 Completed Date: 2009-12-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 405-14 Citation Subset: IM |
Affiliation:
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Department of Legal Medicine, Shinshu University School of Medicine, Matsumoto, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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DNA
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genetics DNA Primers / metabolism DNA Restriction Enzymes / metabolism HLA-DR Antigens / genetics Humans Polymerase Chain Reaction / methods* Polymorphism, Restriction Fragment Length / genetics Polymorphism, Single Nucleotide / genetics Restriction Mapping / methods* Templates, Genetic |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/HLA-DR Antigens; 128338-86-3/HLA-DRB1 antigen; 9007-49-2/DNA; EC 3.1.21.-/DNA Restriction Enzymes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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