Document Detail


Restriction endonuclease profiles of orf virus isolates from the British Isles.
MedLine Citation:
PMID:  9773469     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A comparison of DNA profiles of representative isolates of orf virus, obtained using four different restriction endonucleases (RE), showed that the enzyme EcoRI could be used to discriminate between wild-type virus isolates and vaccine strains. The enzyme was used to compare the RE profiles of orf virus isolates from 43 outbreaks of orf that occurred in vaccinated flocks between 1988 and 1993; 21 outbreaks yielded wild-type virus, 10 yielded vaccine viruses, three produced both vaccine and wild-type viruses and no clear result was obtained from nine of the outbreaks. From the 21 outbreaks yielding wild-type viruses, 28 orf virus isolates had clear RE profiles and 15 distinct RE profiles were recorded. Usually only one virus type was associated with each outbreak but from two farms, two different wild-type viruses were recovered. No predominant genotype was identified, with four RE profile types being recovered for more than one outbreak. From the more severe form of orf involving the buccal cavities of lambs only wild-type viruses were recovered, with at least four different genotypes being represented.
Authors:
J A Gilray; P F Nettleton; I Pow; C J Lewis; S A Stephens; J D Madeley; H W Reid
Related Documents :
9773469 - Restriction endonuclease profiles of orf virus isolates from the british isles.
12698919 - A current review of ebola virus: pathogenesis, clinical presentation, and diagnostic as...
22094079 - The roles of host factors in tombusvirus rna recombination.
22085599 - Measles genotypes d4 and g3 reintroduced by multiple foci after 15 years without measle...
21698129 - Development of an efficient recombinant mosquito densovirus-mediated rna interference s...
22241979 - Eurasian-origin gene segments contribute to the transmissibility, aerosol release, and ...
6309669 - Alteration of rabbit alveolar and peritoneal macrophage function by herpes simplex virus.
2578839 - Amphotropic retrovirus vector transfer of the v-ras oncogene to human hematopoietic and...
22114339 - Inoculation of swine with foot-and-mouth disease sap-mutant virus induces early protect...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Veterinary record     Volume:  143     ISSN:  0042-4900     ISO Abbreviation:  Vet. Rec.     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1998-12-14     Completed Date:  1998-12-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0031164     Medline TA:  Vet Rec     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  237-40     Citation Subset:  IM    
Affiliation:
Moredun Research Institute, International Research Centre, Penicuik, Midlothian.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
DNA Restriction Enzymes / diagnostic use
DNA, Viral / genetics
Ecthyma, Contagious / virology
Genotype
Great Britain
Orf virus / genetics*,  isolation & purification
Sheep / virology
Chemical
Reg. No./Substance:
0/DNA, Viral; EC 3.1.21.-/DNA Restriction Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Opioid responsiveness in patients with advanced head and neck cancer.
Next Document:  Effects of simulated lairage conditions on the physiology and behaviour of pigs.