| Restriction of DNA replication to the reductive phase of the metabolic cycle protects genome integrity. | |
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MedLine Citation:
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PMID: 17600220 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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When prototrophic yeast cells are cultured under nutrient-limited conditions that mimic growth in the wild, rather than in the high-glucose solutions used in most laboratory studies, they exhibit a robustly periodic metabolic cycle. Over a cycle of 4 to 5 hours, yeast cells rhythmically alternate between glycolysis and respiration. The cell division cycle is tightly constrained to the reductive phase of this yeast metabolic cycle, with DNA replication taking place only during the glycolytic phase. We show that cell cycle mutants impeded in metabolic cycle-directed restriction of cell division exhibit substantial increases in spontaneous mutation rate. In addition, disruption of the gene encoding a DNA checkpoint kinase that couples the cell division cycle to the circadian cycle abolishes synchrony of the metabolic and cell cycles. Thus, circadian, metabolic, and cell division cycles may be coordinated similarly as an evolutionarily conserved means of preserving genome integrity. |
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Authors:
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Zheng Chen; Elizabeth A Odstrcil; Benjamin P Tu; Steven L McKnight |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Science (New York, N.Y.) Volume: 316 ISSN: 1095-9203 ISO Abbreviation: Science Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-06-29 Completed Date: 2007-07-10 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0404511 Medline TA: Science Country: United States |
Other Details:
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Languages: eng Pagination: 1916-9 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle* Cell Cycle Proteins / genetics, metabolism DNA Replication* Genome, Fungal* Glycolysis* Homeostasis Hydrogen Peroxide / metabolism Metabolic Networks and Pathways Methionine / metabolism Oxidation-Reduction Protein-Serine-Threonine Kinases / genetics, metabolism Saccharomyces cerevisiae / cytology, genetics*, growth & development, metabolism* Saccharomyces cerevisiae Proteins / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/SML1 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 63-68-3/Methionine; 7722-84-1/Hydrogen Peroxide; EC 2.7.1.-/RAD53 protein, S cerevisiae; EC 2.7.11.1/Protein-Serine-Threonine Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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