Document Detail

Restricting Bmp-4 mediated apoptosis in hindbrain neural crest.
MedLine Citation:
PMID:  11241835     Owner:  NLM     Status:  MEDLINE    
The segregation of the rhombencephalic neural crest into three streams, destined for particular pharyngeal arches, is a prominent feature of the developing vertebrate head and is likely necessary for normal morphogenesis. At least in part, the segregation of the crest into these streams involves the focal depletion of neural crest from rhombomeres 3 and 5 through large-scale apoptosis, mediated by the induction of Bmp-4 expression in the crest primordia of these two rhombomeres. Previously we found that in contrast to rhombomeres 3 and 5, the intervening segment rhombomere 4 was not susceptible to Bmp-4-sponsored neural crest cell death. To analyse the reason for this difference, we have isolated clones for the necessary components of the Bmp-4 signal transduction apparatus. We find that the hindbrain neural crest generally is primed to respond to Bmp-4 and that in vitro, besides rhombomere 3 and 5, rhombomeres 2 and 6 are also sensitive to Bmp-4-sponsored death. As before, however, we find that rhombomere 4 will not respond to this factor. Our analysis of the Bmp-4 antagonists has uncovered a reason for this. Rhombomere 4 expresses elevated levels of the antagonist Noggin at its dorsal midline just as crest production from this segment commences and, as development proceeds, in the crest that migrates away from there. At these later stages, expression also becomes apparent in the migratory post-otic crest as it flows by the otic vesicle. An interesting feature of these domains of Noggin expression is that they lie between territories of Bmp-4 expression, suggesting that Noggin is acting to protect from Bmp-4-mediated cell death.
A Smith; A Graham
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  220     ISSN:  1058-8388     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-03-12     Completed Date:  2001-05-24     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  276-83     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Wiley-Liss, Inc.
MRC Centre for Developmental Neurobiology, Guys Campus, Kings College London, London, England.
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MeSH Terms
Amino Acid Sequence
Apoptosis* / drug effects
Base Sequence
Bone Morphogenetic Protein 4
Bone Morphogenetic Protein Receptors
Bone Morphogenetic Proteins / genetics,  metabolism*,  pharmacology
Carrier Proteins
Chick Embryo
DNA Primers / genetics
DNA-Binding Proteins / genetics,  metabolism
Gene Expression Regulation, Developmental
In Situ Hybridization
Neural Crest / cytology*,  drug effects,  metabolism*
Proteins / genetics,  metabolism
Receptors, Cell Surface / genetics,  metabolism
Receptors, Growth Factor*
Rhombencephalon / drug effects,  embryology*,  metabolism*
Signal Transduction
Smad Proteins
Trans-Activators / genetics,  metabolism
Reg. No./Substance:
0/Bone Morphogenetic Protein 4; 0/Bone Morphogenetic Proteins; 0/Carrier Proteins; 0/DNA Primers; 0/DNA-Binding Proteins; 0/Proteins; 0/Receptors, Cell Surface; 0/Receptors, Growth Factor; 0/Smad Proteins; 0/Trans-Activators; 148294-77-3/noggin protein; EC Morphogenetic Protein Receptors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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