| Restricted wheel access following a light cycle inversion slows re-entrainment without internal desynchrony as measured in Per2 Luc mice. | |
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MedLine Citation:
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PMID: 21392557 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Circadian rhythms are physiological and behavioral oscillations that have period lengths of approximately 24 hours. In mammals, circadian rhythms are driven by a master pacemaker in the hypothalamic suprachiasmatic nucleus (SCN). These rhythms can be entrained to light:dark cycles through photic and non-photic cues. Current research suggests that the SCN re-entrains rapidly to new light:dark (LD) cycles with the first photic cues, whereas peripheral tissues re-entrain more slowly, leading to a transient state of internal disorder while the organism adjusts to the new timing of photic input. To assess internal temporal order during the readjustment we used dim light to slow the rate of re-entrainment following a 12-hour inversion of the LD cycle. We also used a wheel-restriction paradigm, which can block behavioral evidence of re-entrainment. Per2 (Luc) mice were entrained to a 12:12 dim LD cycle with wheel access ad libitum. Following a 12-hour shift in the LD cycle, some animals were subjected to wheel restriction; wheels were locked during the new dark period and available during the new light period. Other mice had wheels available ad lib throughout the experiment. Behavioral actograms of general locomotor activity as measured with motion sensors indicated that mice with ad lib access to wheels were able to re-entrain at a rate significantly faster than mice with restricted wheel access. Up to two weeks following the LD inversion many wheel-restricted animals were still active predominantly in the new light period. Phase of the PER2::LUC bioluminescence rhythms in SCN and four peripheral tissues (lung, esophagus, thymus and spleen), measured ex vivo on days 2, 9 and 16 following the inversion, indicated that within each condition the SCN and peripheral tissues shifted at the same rate, whereas the rate of re-entrainment for the tissues differed between conditions. Ex vivo data showed that the PER2::LUC peak in SCN and peripheral tissues was closely linked to time of activity onset in both groups. Thus, this wheel restriction protocol is capable of reducing and in some cases apparently hindering photic re-entrainment of the circadian system, verifying this protocol as a mechanism for study of photic/non-photic entrainment interactions. Our results suggest that LD inversion under dim light and a wheel-restriction protocol does not induce internal desynchrony, indicating that slowing the rate of shift by limiting both entrainment inputs may induce less "jet lag". |
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Authors:
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Catherine Castillo; Penny Molyneux; Rebecca Carlson; Mary E Harrington |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-7 |
Journal Detail:
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Title: Neuroscience Volume: - ISSN: 1873-7544 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-3-11 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7605074 Medline TA: Neuroscience Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 IBRO. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
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Neuroscience Program, Smith College, Northampton, MA 01063 USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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