Document Detail


Restricted specificity of anti-V3 antibodies induced in humans by HIV candidate vaccines.
MedLine Citation:
PMID:  9390746     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We analyzed the fine specificity of anti-V3 antibodies elicited in three different species (human, guinea pig, and macaque) by various HIV candidate vaccines. Following immunization with recombinant canarypox virus expressing gp160MN or with recombinant gp160MN/LAI, this antibody response was shown to be directed against the NH2-terminal region of the V3 loop. Although this response was increased by a prime-boost regimen using immunization with canarypox expressing gp160 followed by an rgp160 boost, its specificity remained restricted mainly to the recognition of this region of the V3 loop. Pepscan analysis of sera confirmed the results obtained by ELISA and allowed the definition of an immunodominant common binding site for these sera located within the sequence NKRKRIHIGPGR. In contrast to these results, a boost with the V3 peptide was shown to broaden the antibody response and pepscan analysis showed that sera from individuals boosted with the V3 synthetic peptide recognize determinants all along the V3 loop. Similar fine specificity of anti-V3 antibodies was obtained in human, guinea pig, and macaque following immunization by a prime-boost regimen using canarypox recombinants expressing gp160 or gp120 and purified rgp160. In contrast, a V3 synthetic peptide boost stimulated the production of antibodies that recognize multiple epitopes within the V3 loop. Because the induction of antibodies that recognize multiple sites in the V3 loop could be of major importance to neutralize different HIV isolates, these results may have implications for the design and selection of HIV candidate vaccines.
Authors:
E Coëffier; J L Excler; M P Kieny; B Meignier; C Moste; J Tartaglia; G Pialoux; D Salmon-Céron; C Leclerc
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Publication Detail:
Type:  Clinical Trial; Controlled Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  AIDS research and human retroviruses     Volume:  13     ISSN:  0889-2229     ISO Abbreviation:  AIDS Res. Hum. Retroviruses     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1998-01-07     Completed Date:  1998-01-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8709376     Medline TA:  AIDS Res Hum Retroviruses     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1471-85     Citation Subset:  IM; X    
Affiliation:
Unité de Biologie des Régulations Immunitaires, Institut Pasteur, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
AIDS Vaccines / immunology*
Amino Acid Sequence
Animals
Antibodies, Viral / immunology*
Antibody Specificity*
Enzyme-Linked Immunosorbent Assay
Epitope Mapping
Guinea Pigs
HIV Envelope Protein gp120 / chemistry,  immunology*
Humans
Macaca
Molecular Sequence Data
Peptide Fragments / chemistry,  immunology*
Chemical
Reg. No./Substance:
0/AIDS Vaccines; 0/Antibodies, Viral; 0/HIV Envelope Protein gp120; 0/HIV envelope protein gp120 (305-321); 0/Peptide Fragments

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