Document Detail

Restricted receptor segregation into membrane microdomains occurs on human T cells during apoptosis induced by galectin-1.
MedLine Citation:
PMID:  10490978     Owner:  NLM     Status:  MEDLINE    
Galectin-1 induces apoptosis of human thymocytes and activated T cells by an unknown mechanism. Apoptosis is a novel function for a mammalian lectin; moreover, given the ubiquitous distribution of the oligosaccharide ligand recognized by galectin-1, it is not clear how susceptibility to and signaling by galectin-1 is regulated. We have determined that galectin-1 binds to a restricted set of T cell surface glycoproteins, and that only CD45, CD43, and CD7 appear to directly participate in galectin-1-induced apoptosis. To determine whether these specific glycoproteins interact cooperatively or independently to deliver the galectin-1 death signal, we examined the cell surface localization of CD45, CD43, CD7, and CD3 after galectin-1 binding to human T cell lines and human thymocytes. We found that galectin-1 binding resulted in a dramatic redistribution of these glycoproteins into segregated membrane microdomains on the cell surface. CD45 and CD3 colocalized on large islands on apoptotic blebs protruding from the cell surface. These islands also included externalized phosphatidylserine. In addition, the exposure of phosphatidylserine on the surface of galectin-1-treated cells occurred very rapidly. CD7 and CD43 colocalized in small patches away from the membrane blebs, which excluded externalized phosphatidylserine. Receptor segregation was not seen on cells that did not die in response to galectin-1, including mature thymocytes, suggesting that spatial redistribution of receptors into specific microdomains is required for triggering apoptosis.
K E Pace; C Lee; P L Stewart; L G Baum
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  163     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-10-21     Completed Date:  1999-10-21     Revised Date:  2011-09-07    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3801-11     Citation Subset:  AIM; IM    
Department of Pathology, University of California, Los Angeles, School of Medicine 90095, USA.
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MeSH Terms
Antigens, CD*
Antigens, CD43
Antigens, CD45 / metabolism
Antigens, CD7 / metabolism
Apoptosis / immunology*
Cell Line, Transformed
Cell Membrane / chemistry,  metabolism
Galectin 1
Hemagglutinins / metabolism,  physiology*
Membrane Glycoproteins / isolation & purification,  metabolism
Peptide Fragments / chemistry,  isolation & purification,  metabolism*
Phosphatidylserines / metabolism
Protein Binding / immunology
Protein Structure, Tertiary
Receptor Aggregation / immunology
Receptors, Antigen, T-Cell / chemistry,  metabolism*
Sialoglycoproteins / metabolism
Signal Transduction / immunology
T-Lymphocytes / cytology*,  metabolism*
Grant Support
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, CD43; 0/Antigens, CD7; 0/Galectin 1; 0/Hemagglutinins; 0/Membrane Glycoproteins; 0/Peptide Fragments; 0/Phosphatidylserines; 0/Receptors, Antigen, T-Cell; 0/Sialoglycoproteins; 0/UN1 sialoglycoprotein, human; EC, CD45

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