Document Detail

Restricted diffusion of OXPHOS complexes in dynamic mitochondria delays their exchange between cristae and engenders a transitory mosaic distribution.
MedLine Citation:
PMID:  23038773     Owner:  NLM     Status:  Publisher    
Mitochondria are involved in cellular energy supply, signaling and apoptosis. Their ability to fuse and divide provides functional and morphological flexibility and is a key feature in mitochondrial quality maintenance. To study the impact of mitochondrial fusion/fission on the reorganization of inner membrane proteins, OXPHOS complexes in mitochondria of different HeLa cells were tagged with fluorescent proteins (GFP and RFP-HA, respectively), and cells were fused by PEG treatment. Redistribution of the tagged OXPHOS complexes was then followed by means of immuno electron microscopy, two color superresolution fluorescence microscopy and single molecule tracking. In contrast to outer membrane and matrix proteins, which mix fast and homogeneously upon mitochondrial fusion, the mixing of inner membrane proteins was decelerated. Our data suggest that in principle (i) with respect to their composition cristae are preserved during fusion of mitochondria and (ii) cristae with mixed OXPHOS complexes are only slowly and successively formed by restricted diffusion of inner membrane proteins into existing cristae. The resulting transitory mosaic appearance of the inner mitochondrial membrane in terms of composition illuminates mitochondrial heterogeneity and potentially is linked to local differences in function and membrane potential.
Verena Wilkens; Wladislaw Kohl; Karin Busch
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-4
Journal Detail:
Title:  Journal of cell science     Volume:  -     ISSN:  1477-9137     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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