| Restricted amino acid nutrition induces attachment to glass and spreading of Ehrlich ascites tumour cells. | |
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MedLine Citation:
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PMID: 3208913 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ehrlich ascites tumour (EAT) cells were cultured in vitro in Eagle's MEM and Medium 199 with a lowered amino-acid content. Under these conditions EAT cells lose their rounded shape typical of highly malignant cancer cells, and begin to spread on the substratum. The changes in EAT cell morphology are preceded by a decrease in the rate of protein synthesis. These changes were maintained for three days after returning the cells to Eagle's MEM with a normal amino-acid content, but the return to control media did not cause reasumption of growth in the once spread cells. The increase in glucose content (up to five-fold) or the presence of inhibitors of DNA synthesis did not prevent the attachment and flattening of EAT cells in media with a lowered amino acid content. Several possible mechanisms of the influence of restricted amino-acid availability on the changes in EAT cell surface properties are pointed out and the need for study of cancer cell responses to restricted nutrition is discussed. |
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Authors:
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J Kajstura; M Michalik; E Zaporowska-Siwiak; W Korohoda |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society Volume: 26 ISSN: 0239-8508 ISO Abbreviation: Folia Histochem. Cytobiol. Publication Date: 1988 |
Date Detail:
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Created Date: 1989-02-21 Completed Date: 1989-02-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8502651 Medline TA: Folia Histochem Cytobiol Country: POLAND |
Other Details:
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Languages: eng Pagination: 83-9 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, Jan Zurzycki Institute of Molecular Biology, Jagellonian University, Kraków, Poland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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pharmacology* Animals Carcinoma, Ehrlich Tumor / drug therapy, pathology, ultrastructure Cell Adhesion / drug effects* DNA / biosynthesis Dactinomycin / pharmacology Deoxyguanosine / pharmacology Female Mice Mice, Inbred Strains Tumor Cells, Cultured / drug effects*, ultrastructure |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 50-76-0/Dactinomycin; 9007-49-2/DNA; 961-07-9/Deoxyguanosine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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