Document Detail

Restricted amino acid nutrition induces attachment to glass and spreading of Ehrlich ascites tumour cells.
MedLine Citation:
PMID:  3208913     Owner:  NLM     Status:  MEDLINE    
Ehrlich ascites tumour (EAT) cells were cultured in vitro in Eagle's MEM and Medium 199 with a lowered amino-acid content. Under these conditions EAT cells lose their rounded shape typical of highly malignant cancer cells, and begin to spread on the substratum. The changes in EAT cell morphology are preceded by a decrease in the rate of protein synthesis. These changes were maintained for three days after returning the cells to Eagle's MEM with a normal amino-acid content, but the return to control media did not cause reasumption of growth in the once spread cells. The increase in glucose content (up to five-fold) or the presence of inhibitors of DNA synthesis did not prevent the attachment and flattening of EAT cells in media with a lowered amino acid content. Several possible mechanisms of the influence of restricted amino-acid availability on the changes in EAT cell surface properties are pointed out and the need for study of cancer cell responses to restricted nutrition is discussed.
J Kajstura; M Michalik; E Zaporowska-Siwiak; W Korohoda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society     Volume:  26     ISSN:  0239-8508     ISO Abbreviation:  Folia Histochem. Cytobiol.     Publication Date:  1988  
Date Detail:
Created Date:  1989-02-21     Completed Date:  1989-02-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8502651     Medline TA:  Folia Histochem Cytobiol     Country:  POLAND    
Other Details:
Languages:  eng     Pagination:  83-9     Citation Subset:  IM    
Department of Cell Biology, Jan Zurzycki Institute of Molecular Biology, Jagellonian University, Kraków, Poland.
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MeSH Terms
Amino Acids / pharmacology*
Carcinoma, Ehrlich Tumor / drug therapy,  pathology,  ultrastructure
Cell Adhesion / drug effects*
DNA / biosynthesis
Dactinomycin / pharmacology
Deoxyguanosine / pharmacology
Mice, Inbred Strains
Tumor Cells, Cultured / drug effects*,  ultrastructure
Reg. No./Substance:
0/Amino Acids; 50-76-0/Dactinomycin; 9007-49-2/DNA; 961-07-9/Deoxyguanosine

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