Document Detail


Restitution of frog gastric mucosa in vitro: effect of basic fibroblast growth factor.
MedLine Citation:
PMID:  8482448     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Rapid re-epithelialization after superficial gastric mucosal injury is caused by migration of persisting viable epithelial cells. Basic fibroblast growth factor (bFGF) has been reported to enhance the healing of experimental duodenal ulcer, but its mode of action is unclear. The present experiments examine whether an effect of bFGF on restitution might contribute to such healing. METHODS: Paired halves of bullfrog fundic gastric mucosa in Ussing chambers were injured by luminal exposure to 1 mol/L NaCl for 10 minutes. RESULTS: Luminal protamine or suramin, both known to interfere with endogenous bFGF, significantly inhibited electrophysiological recovery at neutral luminal pH (pHL). Luminal sucrose octasulfate, which prevents acid degradation of bFGF, and an exogenous, acid-resistant form of bFGF allowed electrophysiological recovery at a pHL of 3.0 that completely prevented restitution in control tissues. Electrophysiological recovery correlated well with morphological restitution. The presence of endogenous bFGF in normal and restituting bullfrog mucosa was confirmed by positive staining with a monoclonal antibody. CONCLUSIONS: It is concluded that rapid epithelial repair after surface injury is at least in part mediated by bFGF.
Authors:
H Paimela; P J Goddard; K Carter; R Khakee; P L McNeil; S Ito; W Silen
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Gastroenterology     Volume:  104     ISSN:  0016-5085     ISO Abbreviation:  Gastroenterology     Publication Date:  1993 May 
Date Detail:
Created Date:  1993-06-03     Completed Date:  1993-06-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0374630     Medline TA:  Gastroenterology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1337-45     Citation Subset:  AIM; IM    
Affiliation:
Department of Surgery, Beth Israel Hospital, Boston, Massachusetts.
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MeSH Terms
Descriptor/Qualifier:
Animals
Fibroblast Growth Factor 2 / pharmacology*
Gastric Mucosa / injuries,  pathology,  physiology*
Immunohistochemistry
Protamines / pharmacology
Rana catesbeiana
Sucrose / analogs & derivatives,  pharmacology
Suramin / pharmacology
Wound Healing / drug effects*
Grant Support
ID/Acronym/Agency:
AM 15681/AM/NIADDK NIH HHS; CA 42275/CA/NCI NIH HHS; GM 48891/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Protamines; 103107-01-3/Fibroblast Growth Factor 2; 145-63-1/Suramin; 57-50-1/Sucrose; 57680-56-5/sucrose octasulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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