Document Detail


Responses to iron deficiency in Arabidopsis thaliana: the Turbo iron reductase does not depend on the formation of root hairs and transfer cells.
MedLine Citation:
PMID:  7766049     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Arabidopsis thaliana (L.) Heynh. Columbia wild type and a root hair-less mutant RM57 were grown on iron-containing and iron-deficient nutrient solutions. In both genotypes, ferric chelate reductase (FCR) of intact roots was induced upon iron deficiency and followed a Michaelis-Menten kinetic with a Km of 45 and 54 microM FeIII-EDTA and a Vmax of 42 and 33 nmol Fe2+.(g FW)-1.min-1 for the wild type and the mutant, respectively. The pH optimum for the reaction was around pH 5.5. The approximately four fold stimulation of FCR activity was independent of formation of root hairs and/or transfer cells induced by iron deficiency. Iron-deficiency-induced chlorosis and the development of a rigid root habit disappeared when ferric chelate was applied to the leaves, while FCR activity remained unchanged. The time course of the responses to iron deficiency showed that morphological and physiological responses were controlled separately.
Authors:
P R Moog; T A van der Kooij; W Brüggemann; J W Schiefelbein; P J Kuiper
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Planta     Volume:  195     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  1995  
Date Detail:
Created Date:  1995-05-11     Completed Date:  1995-05-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  505-13     Citation Subset:  B    
Affiliation:
Department of Plant Physiology, University of Groningen, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / enzymology,  metabolism*,  ultrastructure
Cells, Cultured
Enzyme Induction
FMN Reductase*
Hydrogen-Ion Concentration
Iron / metabolism*
Kinetics
Microscopy, Electron
NADH, NADPH Oxidoreductases / biosynthesis,  metabolism*
Plant Roots / metabolism
Chemical
Reg. No./Substance:
7439-89-6/Iron; EC 1.5.1.29/FMN Reductase; EC 1.6.-/NADH, NADPH Oxidoreductases; EC 1.6.99.-/ferric citrate iron reductase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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