Document Detail


Responses to DNA damage in Xenopus: cell death or cell cycle arrest.
MedLine Citation:
PMID:  11444046     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Xenopus embryos divide rapidly following fertilization. During this rapid period of cleavage, cell divisions are not sensitive to DNA replication or spindle assembly inhibition. Here, we have investigated the consequences of eliciting DNA damage in these embryos. We show that the rapid cell divisions are not affected by DNA damage. However, as the embryos reach the onset of gastrulation, they undergo rapid and synchronous apoptosis. We have investigated the regulation on this delayed apoptotic response to DNA damage. Next, we have reconstituted a DNA damage cell cycle checkpoint in vitro, demonstrating that all the checkpoint signalling components are present in the embryos but are not activated under the experimental conditions used to generate DNA damage in the embryo.
Authors:
J Greenwood; V Costanzo; K Robertson; C Hensey; J Gautier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.; Review    
Journal Detail:
Title:  Novartis Foundation symposium     Volume:  237     ISSN:  1528-2511     ISO Abbreviation:  Novartis Found. Symp.     Publication Date:  2001  
Date Detail:
Created Date:  2001-07-10     Completed Date:  2002-02-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9807767     Medline TA:  Novartis Found Symp     Country:  England    
Other Details:
Languages:  eng     Pagination:  221-30; discussion 230-4     Citation Subset:  IM    
Affiliation:
Department of Genetics and Development and Department of Dermatology, Columbia University, 630 West 168th Street, New York, NY 10032, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle / physiology*
Cell Death / physiology*
DNA Damage*
Embryo, Nonmammalian / cytology,  physiology*
Fluorescent Dyes / metabolism
Genes, cdc
Green Fluorescent Proteins
In Situ Nick-End Labeling
Luminescent Proteins / genetics,  metabolism
Proteins / genetics,  metabolism
X-Linked Inhibitor of Apoptosis Protein
Xenopus laevis / embryology*,  genetics,  physiology
Zygote / physiology,  radiation effects
Grant Support
ID/Acronym/Agency:
DAMD17-97-1-7071/DA/NIDA NIH HHS; R01GM56781/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Luminescent Proteins; 0/Proteins; 0/X-Linked Inhibitor of Apoptosis Protein; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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