Document Detail

Responses of rat pulp cells to heat stress in vitro.
MedLine Citation:
PMID:  16632756     Owner:  NLM     Status:  MEDLINE    
Although heat stress can cause irritation in the dentin/pulp complex, little is known about the thermotolerance of pulp cells and their response to heat stress. We investigated cultured rat pulp cell responses to heat stress. Cells were subjected to a temperature of 42 degrees C for 30 minutes, and HSPs, alkaline phosphatase activity, and gap-junctional communication were determined at various time points. Although only low levels of HSP70 expression were detected before heat treatment, heat shock markedly induced HSP70 expression, with it gradually increasing at 1 hour after being heated. HSP25, however, showed no dramatic change. Gap junction protein connexin43 rapidly degraded after heat treatment, recovering to normal levels within the following 6 hours. Alkaline phosphatase activity decreased immediately after heat stress, recovering after 1 hour. These results indicate that dental pulp possesses protective factors, including HSPs, and that it can recover viability of intercellular communication and alkaline phosphatase activity after heat stress.
T Amano; T Muramatsu; K Amemiya; K Kubo; M Shimono
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of dental research     Volume:  85     ISSN:  0022-0345     ISO Abbreviation:  J. Dent. Res.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-04-24     Completed Date:  2006-06-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0354343     Medline TA:  J Dent Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  432-5     Citation Subset:  D; IM    
Department of Pathology, Tokyo Dental College, Chiba, Japan.
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MeSH Terms
Alkaline Phosphatase / metabolism
Blotting, Western
Cell Communication
Cell Survival
Cells, Cultured
Connexin 43 / biosynthesis
Connexins / biosynthesis
Dental Pulp / cytology,  metabolism,  physiology*
HSP27 Heat-Shock Proteins
HSP70 Heat-Shock Proteins / biosynthesis
Heat Stress Disorders / physiopathology*
Heat-Shock Proteins / metabolism
Heat-Shock Response / physiology*
Hot Temperature
Neoplasm Proteins / metabolism
Rats, Sprague-Dawley
Reg. No./Substance:
0/Connexin 43; 0/Connexins; 0/HSP27 Heat-Shock Proteins; 0/HSP70 Heat-Shock Proteins; 0/Heat-Shock Proteins; 0/Hspb1 protein, rat; 0/Neoplasm Proteins; EC Phosphatase

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