Document Detail

Responses of metabolic pathways to polycyclic aromatic compounds in flounder following oil spill in the Baltic Sea near the Estonian coast.
MedLine Citation:
PMID:  20663574     Owner:  NLM     Status:  MEDLINE    
In January 2006 an oil spill that involved approximately 40tons of heavy fuel oil affected more than 30km of the north-west coast of Estonia. The aquatic pollution of the coastal area of the Baltic Sea was monitored by measuring the content of selected polycyclic aromatic hydrocarbons (PAHs and PAH metabolites) in flounder (Platichthys flesus trachurus Duncker). One hundred and thirty-one fish were collected: muscle and liver tissues were analyzed by high-performance liquid chromatography (HPLC); bile and urine samples were analyzed using fixed wavelengths fluorescence. Fifteen different types of PAHs were analyzed in liver and muscle, and four types of PAH metabolites were analyzed in bile and urine (2-, 3-, 4- and 5-ringed PAH metabolites represented by naphthalene, phenanthrene, pyrene and benzo(a)pyrene). Fluorescence analyses were carried out using excitation/emission wavelength pairs: 290/380, 256/380, 341/383 and 380/430nm, respectively. There was a time-dependent decrease of PAH concentrations in liver (83%), bile (82%) and urine (113%). HPLC analysis of muscle tissues demonstrated low concentrations of single PAHs, but a decrease of concentrations during the study period was not observed. During the analyses concentrations of PAH metabolites in bile and urine were compared. Liver metabolic transformation activity is believed to exceed that of the kidney but the analyses demonstrated high metabolite concentration in fish urine, particularly of 4- and 5-ring PAH metabolites. The results indicate remarkable buffer capacity of hydrodynamically active sea as well as considerable importance of kidney-urine metabolic pathways in flounder physiology.
Randel Kreitsberg; Irina Zemit; Rene Freiberg; Meelis Tambets; Arvo Tuvikene
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-01
Journal Detail:
Title:  Aquatic toxicology (Amsterdam, Netherlands)     Volume:  99     ISSN:  1879-1514     ISO Abbreviation:  Aquat. Toxicol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-20     Completed Date:  2010-12-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8500246     Medline TA:  Aquat Toxicol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  473-8     Citation Subset:  IM    
Copyright Information:
2010 Elsevier B.V. All rights reserved.
Department of Zoology, Institute of Ecology and Earth Sciences, University of Tartu, Vanemuise 46, 51020 Tartu, Estonia.
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MeSH Terms
Analysis of Variance
Bile / chemistry
Chromatography, High Pressure Liquid
Environmental Monitoring / statistics & numerical data*
Flounder / metabolism*
Kidney / metabolism
Liver / chemistry,  metabolism
Metabolic Networks and Pathways / drug effects*
Muscle, Skeletal / chemistry
Oceans and Seas
Polycyclic Hydrocarbons, Aromatic / analysis,  pharmacokinetics,  toxicity*,  urine
Water Pollutants, Chemical / analysis,  pharmacokinetics,  toxicity*,  urine
Reg. No./Substance:
0/Petroleum; 0/Polycyclic Hydrocarbons, Aromatic; 0/Water Pollutants, Chemical

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