Document Detail


Responses of alkaline phosphatase activity to phosphorus stress in Daphnia magna.
MedLine Citation:
PMID:  20038659     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We examined how alkaline phosphatase (AP) activity within the bodies and in the materials released by the crustacean Daphnia magna responds to variable algal food phosphorus (P)-content. We found that Daphnia eating P-poor food (C:P approximately 700) had significantly higher AP activity in their bodies on a mass-specific basis compared with individuals eating P-rich food (C:P approximately 100). This dietary P effect on AP activity was not altered by Daphnia starvation but was partially related to differences in the P concentration of animal body homogenates. By contrast, poor P-nutrition of Daphnia lowered AP activity in released materials compared with that measured from their P-sufficient conspecifics. Moreover, AP activity in Daphnia release was lowest in animals consuming P-poor food for longer time periods. Our results support the hypothesis that AP activity increases inside P-limited Daphnia as a mechanism to increase P-acquisition and retention from ingested algae in these nutritionally stressed animals. The lower level of AP activity present in the water of P-deprived animals could reflect a change from largely free to membrane-bound AP isotypes in the digestive tracts of P-starved animals or a decrease in the shedding of membrane-anchored AP from their intestinal lining. These results supplement accumulating evidence that P-poor algal food reduces the dietary mineral P available to Daphnia. In addition, animal body AP activity measurements, with some refinement, may prove useful as an in situ indicator of P-stress in aquatic consumers.
Authors:
S D S McCarthy; S P Rafferty; P C Frost
Related Documents :
19094259 - The principles, practices and some future applications of near infrared spectroscopy fo...
8457919 - Heritability and diagnosis of congenital abnormalities in food animals.
17715819 - Application of campylobacter molecular classification and typing techniques in veterina...
12052189 - Pf1022a and related cyclodepsipeptides - a novel class of anthelmintics.
7349769 - Harmful effects of carrageenan fed to animals.
3559859 - The role of intraluminal tension and ph in the development of necrotizing enterocolitis...
22874429 - Assessment of the daily intake of 62 polychlorinated biphenyls from dietary exposure in...
18178249 - High dose cart peptide induces abnormal eeg activity and behavioral seizures.
16376969 - Estimation of 1999-2004 dietary daily intake of pcdds, pcdfs and dioxin-like pcbs by a ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of experimental biology     Volume:  213     ISSN:  1477-9145     ISO Abbreviation:  J. Exp. Biol.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-12-29     Completed Date:  2010-03-23     Revised Date:  2010-05-19    
Medline Journal Info:
Nlm Unique ID:  0243705     Medline TA:  J Exp Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  256-61     Citation Subset:  IM    
Affiliation:
Department of Biology, Trent University, Peterborough, ON, Canada K9J 7B8.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / metabolism*
Animal Feed
Animals
Daphnia / drug effects*,  enzymology*
Energy Metabolism
Phosphorus, Dietary / pharmacology*
Chemical
Reg. No./Substance:
0/Phosphorus, Dietary; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Spatial vision in the purple sea urchin Strongylocentrotus purpuratus (Echinoidea).
Next Document:  The moment before touchdown: landing manoeuvres of the honeybee Apis mellifera.