| Responses of Staphylococcus aureus exposed to HCl and organic acid stress. | |
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MedLine Citation:
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PMID: 20921988 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Staphylococcus aureus is an important food poisoning bacterium. In food preservation, acidification is a well-known method. Permeant weak organic acids, like lactic and acetic acids, are known to be more effective against bacteria than inorganic strong acids (e.g., HCl). Growth experiments and metabolic and transcriptional analyses were used to determine the responses of a food pathogenic S. aureus strain exposed to lactic acid, acetic acid, and HCl at pH 4.5. Lactic and acetic acid stress induced a slower transcriptional response and large variations in growth patterns compared with the responses induced by HCl. In cultures acidified with lactic acid, the pH of the medium gradually increased to 7.5 during growth, while no such increase was observed for bacteria exposed to acetic acid or HCl. Staphylococcus aureus increased the pH in the medium mainly through accumulation of ammonium and the removal of acid groups, resulting in increased production of diacetyl (2,3-butanedione) and pyrazines. The results showed flexible and versatile responses of S. aureus to different types of acid stress. As measured by growth inhibition, permeant organic acid stress introduced severe stress compared with the stress caused by HCl. Cells exposed to lactic acid showed specific mechanisms of action in addition to sharing many of the mechanisms induced by HCl stress. |
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Authors:
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Tone Mari Rode; Trond Møretrø; Solveig Langsrud; Oyvind Langsrud; Gjermund Vogt; Askild Holck |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Canadian journal of microbiology Volume: 56 ISSN: 1480-3275 ISO Abbreviation: Can. J. Microbiol. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-10-05 Completed Date: 2010-12-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372707 Medline TA: Can J Microbiol Country: Canada |
Other Details:
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Languages: eng Pagination: 777-92 Citation Subset: IM |
Affiliation:
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Nofima Mat AS, Osloveien 1, N-1430 Ås, Norway. tone.mari.rode@nofima.no |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetic Acid
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pharmacology* Acids / pharmacology Adaptation, Physiological Ammonia / metabolism Citric Acid Cycle Food Microbiology Gene Expression Regulation, Bacterial* Gene Expression Regulation, Enzymologic Hydrochloric Acid / pharmacology* Hydrogen-Ion Concentration Lactic Acid / pharmacology* Oxidative Stress Pentose Phosphate Pathway Polymerase Chain Reaction Pyruvic Acid / metabolism Staphylococcus aureus / genetics, growth & development, physiology* Stress, Physiological* |
| Chemical | |
Reg. No./Substance:
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0/Acids; 127-17-3/Pyruvic Acid; 50-21-5/Lactic Acid; 64-19-7/Acetic Acid; 7647-01-0/Hydrochloric Acid; 7664-41-7/Ammonia |
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