Document Detail


Response to experimentally induced infection with bovine respiratory syncytial virus following intranasal vaccination of seropositive and seronegative calves.
MedLine Citation:
PMID:  20433400     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To determine whether a combination modified-live bovine respiratory syncytial virus (BRSV) vaccine can stimulate protective immunity in young BRSV-seropositive calves following intranasal (IN) administration. DESIGN: Controlled challenge study. ANIMALS: 66 Holstein bull calves, 3 to 8 days old. PROCEDURES: In experiment 1, BRSV-seropositive and -seronegative calves were vaccinated IN with a commercially available combination modified-live virus vaccine formulated for SC administration; calves underwent BRSV challenge 4.5 months later. In experiment 2, BRSV-seronegative calves were vaccinated IN or SC (to examine the effect of route of administration) with the same combination vaccine that instead had a 1/100 dose of BRSV (to examine the effect of dose); calves underwent BRSV challenge 21 days later. RESULTS: In experiment 1, BRSV challenge resulted in severe respiratory tract disease with low arterial partial pressures of oxygen and lung lesions in most calves from all groups. Maximum change in rectal temperature was significantly greater in seropositive IN vaccinated calves, compared with seronegative IN vaccinated and seropositive control calves. Number of days of BRSV shedding was significantly lower in seronegative IN vaccinated calves than in seropositive IN vaccinated and seropositive control calves. In experiment 2, maximum change in rectal temperature was significantly greater in seronegative control calves, compared with seronegative IN and SC vaccinated calves. Shedding of BRSV was significantly reduced in seronegative IN and SC vaccinated calves, compared with control calves; also, lung lesions were reduced in seronegative IN and SC vaccinated calves. CONCLUSIONS AND CLINICAL RELEVANCE: Maternal antibodies may inhibit priming of protective responses by IN delivered BRSV vaccines.
Authors:
John A Ellis; Sheryl P Gow; Noriko Goji
Publication Detail:
Type:  Controlled Clinical Trial; Journal Article    
Journal Detail:
Title:  Journal of the American Veterinary Medical Association     Volume:  236     ISSN:  0003-1488     ISO Abbreviation:  J. Am. Vet. Med. Assoc.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-03     Completed Date:  2010-05-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7503067     Medline TA:  J Am Vet Med Assoc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  991-9     Citation Subset:  IM    
Affiliation:
Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada. john.ellis@usask.ca
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MeSH Terms
Descriptor/Qualifier:
Administration, Intranasal
Animals
Antibodies, Viral / blood
Cattle
Cattle Diseases / prevention & control*,  virology
Lung / pathology
Male
Nose / virology
Pneumonia, Viral / pathology,  veterinary
Respiratory Syncytial Virus Infections / prevention & control,  veterinary*
Respiratory Syncytial Virus, Bovine*
Viral Vaccines / administration & dosage,  immunology*
Virus Shedding
Chemical
Reg. No./Substance:
0/Antibodies, Viral; 0/Viral Vaccines

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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