Document Detail


Response of sugar interconversion, starch and protein accumulation to supplied metabolites during pod filling in chickpea.
MedLine Citation:
PMID:  11233085     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Detached chickpea inflorescences bearing pods at 20 days after flowering (DAF) were cultured for 5 days in complete liquid medium supplemented separately with asparate, myo-inositol, alpha-ketoglutarate and phytic acid. Effect of these metabolites on sugar interconvestion and starch and protein accumulation in developing pods was studied. Substituting asparate (62.5 mM) for glutamine in culture medium decreased relative proportion of sucrose in all pod tissues but increased the level of sugars, starch and protein in pod wall and cotyledons. In cotyledons, whereas myo-inositol (75 mM) reduced the accumulation of starch without affecting protein level, alpha-ketoglutarate (44 mM) increased both starch and protein accumulation. Both myo-inositol and alpha-ketoglutarate increased relative proportion of sucrose in cotyledons. Phytic acid (1 mM) decreased in cotyledons 14C incorporation from glucose into EtOH extract (principally constituted by sugars), amino acids and proteins but increased the same into starch. In cotyledons, phytic acid also increased 14C incorporation from glutamate into amino acids but this increase was negatively correlated with protein synthesis. Phytic acid decreased the relative distribution of 14C from glucose and glutamate into sucrose from pod wall but enhanced the same into EtOH extract from embryo. Based on the results, it is suggested that mode of metabolic response to exogenously supplied metabolites widely differs in pod tissues of chickpea.
Authors:
R Singh; B Kaushal
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Indian journal of experimental biology     Volume:  38     ISSN:  0019-5189     ISO Abbreviation:  Indian J. Exp. Biol.     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2001-03-06     Completed Date:  2001-05-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0233411     Medline TA:  Indian J Exp Biol     Country:  India    
Other Details:
Languages:  eng     Pagination:  51-5     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Punjab Agricultural University, Ludhiana 141004, India.
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MeSH Terms
Descriptor/Qualifier:
Aspartic Acid / metabolism,  pharmacology
Carbohydrate Metabolism*
Culture Media / pharmacology
Fabaceae / metabolism*
Humans
Inositol / metabolism,  pharmacology
Ketoglutaric Acids / metabolism,  pharmacology
Phytic Acid / metabolism,  pharmacology
Plant Proteins / metabolism*
Plants, Medicinal*
Seeds / metabolism*
Starch / metabolism*
Chemical
Reg. No./Substance:
0/Culture Media; 0/Ketoglutaric Acids; 0/Plant Proteins; 328-50-7/alpha-ketoglutaric acid; 56-84-8/Aspartic Acid; 6917-35-7/Inositol; 83-86-3/Phytic Acid; 9005-25-8/Starch

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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