| Response of monocytes exposed to phagocytosable particles and discs of comparable surface roughness. | |
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MedLine Citation:
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PMID: 17631956 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This in vitro study characterized the temporal cytokine expression profile from human monocytes exposed to phagocytosable Ti particles (0.78+/-0.12 microm) and to Ti discs of comparable surface roughness. Human THP-1 monocytes were cultured in six well tissue culture polystyrene (TCPS) plates. Each well was either bare, contained Ti particles (the particles were clearly engulfed by the monocytes), or contained a Ti disc. Half of the wells were treated with 1 microg/mL lipopolysaccharide (LPS), while the other half were left unstimulated. Unstimulated and LPS-stimulated cells in bare wells were the negative and positive controls, respectively. Supernatant was sampled from each well at 1, 6, 24, 48, and 72 h and assayed for the expression of nine different cytokines using a Luminex system. Three cytokines (IL-1beta, GM-CSF and IL-13) gave little to no response under all conditions, while six cytokines (TNF-alpha, IL-6, MIP-1alpha, MCP-1, VEGF, and IL-1ra) were clearly detectable. Expression levels generally increased with culture time, particle concentration, and LPS stimulation. Most significantly, it was found that cells treated by Ti discs produced in many instances a higher cytokine expression than did particles. |
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Authors:
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Dong-Hwan Kim; Matt T Novak; Jamie Wilkins; Minkyu Kim; Anita Sawyer; William M Reichert |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2007-07-12 |
Journal Detail:
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Title: Biomaterials Volume: 28 ISSN: 0142-9612 ISO Abbreviation: Biomaterials Publication Date: 2007 Oct |
Date Detail:
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Created Date: 2007-08-13 Completed Date: 2007-10-19 Revised Date: 2012-04-13 |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 4231-9 Citation Subset: IM |
Affiliation:
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Department of Biomedical Engineering, Duke University, 136 Hudson Hall, Box 90281, Durham, NC 27708, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line Cytokines / immunology* Humans Materials Testing Monocytes / drug effects, immunology* Nanoparticles / administration & dosage*, chemistry* Particle Size Phagocytosis / drug effects*, immunology Surface Properties Titanium / administration & dosage*, chemistry* |
| Grant Support | |
ID/Acronym/Agency:
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DK054932/DK/NIDDK NIH HHS; R01 DK054932-09/DK/NIDDK NIH HHS; R01 DK054932-13/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 7440-32-6/Titanium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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