Document Detail


Response of monocytes exposed to phagocytosable particles and discs of comparable surface roughness.
MedLine Citation:
PMID:  17631956     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This in vitro study characterized the temporal cytokine expression profile from human monocytes exposed to phagocytosable Ti particles (0.78+/-0.12 microm) and to Ti discs of comparable surface roughness. Human THP-1 monocytes were cultured in six well tissue culture polystyrene (TCPS) plates. Each well was either bare, contained Ti particles (the particles were clearly engulfed by the monocytes), or contained a Ti disc. Half of the wells were treated with 1 microg/mL lipopolysaccharide (LPS), while the other half were left unstimulated. Unstimulated and LPS-stimulated cells in bare wells were the negative and positive controls, respectively. Supernatant was sampled from each well at 1, 6, 24, 48, and 72 h and assayed for the expression of nine different cytokines using a Luminex system. Three cytokines (IL-1beta, GM-CSF and IL-13) gave little to no response under all conditions, while six cytokines (TNF-alpha, IL-6, MIP-1alpha, MCP-1, VEGF, and IL-1ra) were clearly detectable. Expression levels generally increased with culture time, particle concentration, and LPS stimulation. Most significantly, it was found that cells treated by Ti discs produced in many instances a higher cytokine expression than did particles.
Authors:
Dong-Hwan Kim; Matt T Novak; Jamie Wilkins; Minkyu Kim; Anita Sawyer; William M Reichert
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-07-12
Journal Detail:
Title:  Biomaterials     Volume:  28     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-08-13     Completed Date:  2007-10-19     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  4231-9     Citation Subset:  IM    
Affiliation:
Department of Biomedical Engineering, Duke University, 136 Hudson Hall, Box 90281, Durham, NC 27708, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Cytokines / immunology*
Humans
Materials Testing
Monocytes / drug effects,  immunology*
Nanoparticles / administration & dosage*,  chemistry*
Particle Size
Phagocytosis / drug effects*,  immunology
Surface Properties
Titanium / administration & dosage*,  chemistry*
Grant Support
ID/Acronym/Agency:
DK054932/DK/NIDDK NIH HHS; R01 DK054932-09/DK/NIDDK NIH HHS; R01 DK054932-13/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Cytokines; 7440-32-6/Titanium
Comments/Corrections

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