| Response of mitochondrial reactive oxygen species generation to steady-state oxygen tension: implications for hypoxic cell signaling. | |
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MedLine Citation:
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PMID: 16963616 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mitochondria are proposed to play an important role in hypoxic cell signaling. One currently accepted signaling paradigm is that the mitochondrial generation of reactive oxygen species (ROS) increases in hypoxia. This is paradoxical, because oxygen is a substrate for ROS generation. Although the response of isolated mitochondrial ROS generation to [O(2)] has been examined previously, such investigations did not apply rigorous control over [O(2)] within the hypoxic signaling range. With the use of open-flow respirometry and fluorimetry, the current study determined the response of isolated rat liver mitochondrial ROS generation to defined steady-state [O(2)] as low as 0.1 microM. In mitochondria respiring under state 4 (quiescent) or state 3 (ATP turnover) conditions, decreased ROS generation was always observed at low [O(2)]. It is concluded that the biochemical mechanism to facilitate increased ROS generation in response to hypoxia in cells is not intrinsic to the mitochondrial respiratory chain alone but may involve other factors. The implications for hypoxic cell signaling are discussed. |
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Authors:
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David L Hoffman; Jason D Salter; Paul S Brookes |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2006-09-08 |
Journal Detail:
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Title: American journal of physiology. Heart and circulatory physiology Volume: 292 ISSN: 0363-6135 ISO Abbreviation: Am. J. Physiol. Heart Circ. Physiol. Publication Date: 2007 Jan |
Date Detail:
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Created Date: 2007-01-10 Completed Date: 2007-02-20 Revised Date: 2007-12-03 |
Medline Journal Info:
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Nlm Unique ID: 100901228 Medline TA: Am J Physiol Heart Circ Physiol Country: United States |
Other Details:
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Languages: eng Pagination: H101-8 Citation Subset: IM |
Affiliation:
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Box 604 Anesthesiology, Univ. of Rochester Medical Center, 601 Elmwood Ave., Rochester, NY 14642, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological
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physiology Animals Cell Hypoxia / physiology* Cell Respiration / physiology* Cells, Cultured Male Mitochondria, Liver / metabolism* Mitochondrial Proteins / metabolism* Oxygen / metabolism* Rats Rats, Sprague-Dawley Reactive Oxygen Species / metabolism* Signal Transduction |
| Grant Support | |
ID/Acronym/Agency:
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HL-71158/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Mitochondrial Proteins; 0/Reactive Oxygen Species; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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