| Response of midgut epithelial cells to Cry1Aa is toxin-dependent and depends on the interplay between toxic action and the host apoptotic response. | |
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MedLine Citation:
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PMID: 22260394 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Cry1Aa is an insecticidal protein produced by Bacilllus thuringiensis. To elucidate the mechanisms of cell/individual death and healing in the midgut epithelium, Bombyx mori larva were administered different concentrations of Cry1Aa and sections from the midgut were examined by TUNEL staining. In the lethal condition, most midgut columnar cells were observed to swell and burst without being stained by TUNEL 4 h after intoxication and the epithelial layer collapsed by 24 h. In the sub-lethal to non-lethal conditions, midgut columnar cells did not swell but their nuclei were intensely stained by TUNEL from 4 to 24 h after intoxication. These apoptotic cells dropped out of the epithelial layer and the epithelial layer was finally renewed by newly mature columnar cells within 72 h after intoxication. These results suggest that the reaction, which occurs in the midgut of intoxicated insects, is determined by the interplay between passive toxic action and the active host healing response, in a toxin concentration-dependent manner. To investigate the signal pathways regulating apoptosis and the proliferation of the intoxicated epithelial layer, the expression of several genes was further examined by quantitative real-time polymerase chain reaction. Bm-Apaf1 was the most up-regulated gene post-intoxication. |
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Authors:
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Shiho Tanaka; Yasutaka Yoshizawa; Ryoichi Sato |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-19 |
Journal Detail:
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Title: The FEBS journal Volume: - ISSN: 1742-4658 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101229646 Medline TA: FEBS J Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Journal compilation © 2012 Federation of European Biochemical Societies. |
Affiliation:
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Graduate School of Bio-Application and Systems Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo, 184-8588, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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