Document Detail


Response of the meadow vole (Microtus pennsylvanicus) to experimental inoculation with Borrelia burgdorferi.
MedLine Citation:
PMID:  7933285     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The response of the meadow vole (Microtus pennsylvanicus) to infection by experimental inoculation with Borrelia burgdorferi was evaluated. Forty-two adult voles were inoculated subcutaneously with 0.5 x 10(6) spirochetes. Sera taken during the 196 day trial were tested by indirect fluorescent antibody (IFA) assay for antibodies to B. burgdorferi. Tissues from animals which died during the trial, and from animals killed at 28, 112 and 196 days post-inoculation (DPI), respectively, were cultured in BSK-II medium for < or = 6 weeks. They also were examined histologically for lesions and the presence of spirochetes. All inoculated animals developed antibodies by 14 DPI and maintained titers > or = 1:10 for the duration of the trial. Spirochetes were isolated from ears, bladder, and spleen. Spirochetes also were identified by Bosma-Steiner silver stain or tissue IFA assay in sections of ears, bladder, kidney and heart. Infection as confirmed by re-isolation persisted for < or = 111 days. No lesions were identified in association with the presence of spirochetes. No increase in mortality was observed in inoculated animals compared with controls. Sensitivity of the IFA test at a cut-off titer of 1:10 was 100% from > or = 14 DPI, but at 1:20 reached a maximum of 97%. Specificity at 1:10 was 84% and at 1:20 was 97%. Use of antiserum to Microtus immunoglobulin (Ig) in a double-layered test provided no significant advantages over use of a commercial fluorescein-conjugated anti-mouse Ig in a single-layered IFA test.
Authors:
G D Campbell; I K Barker; R P Johnson; P E Shewen; S A McEwen; G A Surgeoner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of wildlife diseases     Volume:  30     ISSN:  0090-3558     ISO Abbreviation:  J. Wildl. Dis.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-11-21     Completed Date:  1994-11-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0244160     Medline TA:  J Wildl Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  408-16     Citation Subset:  IM    
Affiliation:
Canadian Cooperative Wildlife Health Centre, Department of Pathology, Ontario Veterinary College, University of Guelph.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Bacterial / blood*
Arvicolinae*
Borrelia burgdorferi Group / immunology*,  isolation & purification
Ear / microbiology
Fluorescent Antibody Technique / veterinary
Kidney / microbiology
Lyme Disease / diagnosis,  immunology,  microbiology,  veterinary*
Rodent Diseases / diagnosis,  immunology*,  microbiology
Sensitivity and Specificity
Silver Staining
Spleen / microbiology
Urinary Bladder / microbiology
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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