| Response of interleukin-1beta in the magnocellular system to salt-loading. | |
| | |
MedLine Citation:
|
PMID: 17076768 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Drinking 2% NaCl decreases interleukin (IL)-1beta in the neural lobe and enhances IL-1 Type 1 receptor expression in magnocellular neurones and pituicytes. To quantify cytokine depletion from the neural lobe during progressive salt loading and determine whether the changes are reversible and correspond with stores of vasopressin (VP) or oxytocin (OT), rats were given water on day 0 and then 2% NaCl to drink for 2, 5, 8 or 5 days followed by 5 days of water (rehydration). Control rats drinking only water were pair-fed amounts eaten by 5-day salt-loaded animals. Animals were decapitated on day 8, the neural lobe frozen and plasma hormones analysed by radioimmunoassay (OT, VP) or enzyme-linked immunosorbent assay (IL-1beta). IL-1beta, VP and OT in homogenates of the neural lobe were quantified by immunocapillary electrophoresis with laser-induced fluorescence detection. Differences were determined by ANOVA, Tukey's t-test, Dunnett's procedure, Fisher's least significant difference and linear regression analysis. In response to salt-loading, rats lost body weight similar to pair-fed controls, drank progressively more 2% NaCl and excreted greater urine volumes. Plasma VP increased at days 2 and 8 of salt-loading, whereas osmolality, OT and cytokine were enhanced after 8 days with IL-1beta remaining elevated after rehydration. In the neural lobe, all three peptides decreased progressively with increasing duration of salt-loading (IL-1beta, r2 = 0.98; OT, r2 = 0.94; VP, r2 = 0.93), beginning on day 2 (IL-1beta; VP) or 5 (OT), with only VP replenished by rehydration. IL-1beta declined more closely (P < 0.0001; ANOVA interaction analysis) with OT (r2 = 0.96) than VP (r2 = 0.86), indicative of corelease from the neural lobe during chronic dehydration. Local effects of IL-1beta on magnocellular terminals, pituicytes and microglia in the neural lobe with activation of forebrain osmoregulatory structures by circulating cytokine may sustain neurosecretion of OT and VP during prolonged salt-loading. |
| | |
Authors:
|
J Y Summy-Long; S Hu; A Pruss; X Chen; T M Phillips |
Related Documents
:
|
8563698 - The role of kinins and atrial natriuretic peptide on the renal effects of neutral endop... 21873448 - Phophatidylinositol-4,5-bisphosphate stimulates alveolar epithelial fluid clearance in ... 17324138 - Involvement of the endothelin et(b) receptor in gender differences in deoxycorticostero... 16554088 - Role of the central opioid system in the inhibition of water and salt intake induced by... 1348588 - Serum and tissue transglutaminase correlates with the severity of inflammation in induc... 21077258 - Antagonistic effects of lycium barbarum polysaccharides on the impaired reproductive sy... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
|
Title: Journal of neuroendocrinology Volume: 18 ISSN: 0953-8194 ISO Abbreviation: J. Neuroendocrinol. Publication Date: 2006 Dec |
Date Detail:
|
Created Date: 2006-11-01 Completed Date: 2007-01-30 Revised Date: 2007-12-03 |
Medline Journal Info:
|
Nlm Unique ID: 8913461 Medline TA: J Neuroendocrinol Country: England |
Other Details:
|
Languages: eng Pagination: 926-37 Citation Subset: IM |
Affiliation:
|
Department of Pharmacology, Penn State College of Medicine, Hershey, PA 17033, USA. jsl2@psu.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Analysis of Variance Animals Dehydration / blood, chemically induced Interleukin-1beta / blood* Male Osmolar Concentration Oxytocin / blood* Pituitary Gland, Posterior / cytology, drug effects, metabolism* Pituitary Hormones, Posterior / blood Rats Rats, Sprague-Dawley Sodium Chloride, Dietary Statistics, Nonparametric Vasopressins / blood* Water-Electrolyte Balance / drug effects, physiology* |
| Grant Support | |
ID/Acronym/Agency:
|
R01-MH65271/MH/NIMH NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Interleukin-1beta; 0/Pituitary Hormones, Posterior; 0/Sodium Chloride, Dietary; 11000-17-2/Vasopressins; 50-56-6/Oxytocin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Long-term voluntary exercise and the mouse hypothalamic-pituitary-adrenocortical axis: impact of con...
Next Document: Ovarian steroids but not the locus coeruleus regulate stress-induced prolactin secretion in female r...