Document Detail

Response of hepatic mitochondrial and peroxisomal beta-oxidation to increasing palmitate concentrations in piglets.
MedLine Citation:
PMID:  9395839     Owner:  NLM     Status:  MEDLINE    
Responses of total, mitochondrial, and peroxisomal beta-oxidation to increasing [1-14C]-palmitate concentrations (0.02-1.0 mM) were measured in liver homogenates from neonatal pigs. Incubations were conducted in the absence (total beta-oxidation) or presence (peroxisomal beta-oxidation) of antimycin A and rotenone; mitochondrial beta-oxidation was calculated as total minus peroxisomal oxidation. Total and mitochondrial beta-oxidations were maximized at a palmitate concentration of 0.05 mM, whereas peroxisomal beta-oxidation was maximized at 0.50 mM palmitate. Across concentrations, peroxisomal beta-oxidation contributed 40-47% of total beta-oxidation. An increased rate of CO2 production and a greater ratio of CO2 production to total mitochondrial beta-oxidation as palmitate concentration increased suggested that the limited capacity for mitochondrial beta-oxidation was attributable primarily to limited ketogenic capacity. Comparative observations in liver from adult rats showed that peroxisomal beta-oxidation was maximized at 0.1 mM palmitate, but total and mitochondrial beta-oxidation rates were not maximized even at 1 mM palmitate. At 1 mM palmitate, peroxisomal beta-oxidation was 20% of total beta-oxidation in adult rats and 37% in adult pigs. Therefore, the contribution of peroxisomal beta-oxidation to total beta-oxidation is highly dependent on substrate concentration and appears to be greater in adult pigs than in adult rats. The greater proportional contribution of peroxisomal beta-oxidation in piglet liver might act as a compensatory mechanism for piglets to oxidize milk fatty acids.
X X Yu; J K Drackley; J Odle; X Lin
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biology of the neonate     Volume:  72     ISSN:  0006-3126     ISO Abbreviation:  Biol. Neonate     Publication Date:  1997  
Date Detail:
Created Date:  1998-02-03     Completed Date:  1998-02-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0247551     Medline TA:  Biol Neonate     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  284-92     Citation Subset:  IM    
Division of Nutritional Sciences, University of Illinois, Urbana 61801, USA.
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MeSH Terms
Animals, Newborn / metabolism*
Carbon Dioxide / metabolism
Dose-Response Relationship, Drug
Liver / drug effects,  metabolism*
Microbodies / drug effects,  metabolism*
Mitochondria, Liver / drug effects,  metabolism*
Osmolar Concentration
Palmitates / pharmacology*
Rats, Sprague-Dawley
Reg. No./Substance:
0/Palmitates; 124-38-9/Carbon Dioxide

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