Document Detail

Response of cyt a,a3 in the situ canine heart to transient ischemic episodes.
MedLine Citation:
PMID:  6268057     Owner:  NLM     Status:  MEDLINE    
Experiments were performed to examine the response of cyt a,a3 to transient ischemic and hypoxic episodes in the empty, fibrillating canine heart in situ. Using a dual wavelength, differential spectrophotometer, reaction spectra show an absorption peak at approximately 605 nm consistent with that obtained from purified cyt a,a3. The characteristics of the averaged reaction spectrum in the interval 590 nm to 610 nm indicate that hemoglobin/myoglobin contribute no more than 23% to the signal measured at 605 nm. A regimen of one 30 sec global ischemia (GI) repeated once every 3 minutes over a 90 min period showed no appreciable signal deterioration. Therefore, five such interventions were subsequently used as the test perturbation. Studies of the effects of ischemic episodes of 30 and 60 min show that the response of cyt a,a3 to this test intervention was smaller (90 +/- 6% and 89 +/- 7%) than that observed prior to the ischemic episode. Changes in coronary perfusion pressure (+/- 10 Torr) produced an immediate oxidation/reduction of cyt a,a3. In the working heart, just prior to fibrillation, 6 sec to interrupted ventilation resulted in a continuous reduction of cyt a,a3. The data from these studies show: 1) The redox state of cyt a,a3 may be continuously monitored in the canine heart in situ. 2) Following ischemias of 30 and 60 min duration, respiratory chain function may be impaired; and 3) The well-perfused epicardium is extremely sensitive to small changes in oxygen delivery.
T R Snow; L H Kleinmann; J C LaManna; A S Wechsler; F F Jöbsis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Basic research in cardiology     Volume:  76     ISSN:  0300-8428     ISO Abbreviation:  Basic Res. Cardiol.     Publication Date:    1981 May-Jun
Date Detail:
Created Date:  1981-10-25     Completed Date:  1981-10-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0360342     Medline TA:  Basic Res Cardiol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  289-304     Citation Subset:  IM    
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MeSH Terms
Coronary Disease / metabolism*
Electron Transport Complex IV / metabolism*
Myocardium / metabolism*
Grant Support
Reg. No./Substance:
EC Transport Complex IV

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