Document Detail

Response of complement and neutrophils to hydrophilized synthetic membranes.
MedLine Citation:
PMID:  3196529     Owner:  NLM     Status:  MEDLINE    
Membranes prepared from hydrophobic synthetic polymers adsorb proteins. Alloying these polymers with hydrophilic polymers increases water wetability and reduces hydrophobic interactions. The authors tested the hypothesis that alloying could affect the biocompatibility of the resultant membrane. Polymer films were cast from solutions containing 12% polysulfone and 4, 8, or 12% polyvinylpyrrolidone in dimethylacetamide. The effect of the films on the complement system and neutrophil function was assessed by incubating them in normal plasma and whole blood at 37 degrees C. Complement activation was followed by measuring plasma concentrations of C3a des Arg and C5a des Arg. Flow cytometric measurements of phagocytic capacity, H2O2 production, and expression of the C3bi receptor were used to assess neutrophil function. The degree of complement activation was found to depend on the composition of the polymer film. C3a des Arg and C5a des Arg generation decreased 75% and 86%, respectively, as the polyvinylpyrolidone content of the casting solution was increased from 25 to 50%. This was accompanied by a decrease in stimulation of neutrophil H2O2 production and C3bi receptor expression. From these data, the authors conclude that alloying with polyvinylpyrrolidone can affect the biocompatibility of polysulfone.
R A Ward; E Klein; G B Harding; K E Murchison
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  ASAIO transactions / American Society for Artificial Internal Organs     Volume:  34     ISSN:  0889-7190     ISO Abbreviation:  ASAIO Trans     Publication Date:    1988 Jul-Sep
Date Detail:
Created Date:  1989-01-26     Completed Date:  1989-01-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8611947     Medline TA:  ASAIO Trans     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  334-7     Citation Subset:  IM    
Division of Neprology, School of Medicine, University of Louisville, Kentucky 40292.
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MeSH Terms
Biocompatible Materials
Complement Activation*
Flow Cytometry
Membranes, Artificial*
Neutrophils* / immunology
Grant Support
1 R43 DK36762-01/DK/NIDDK NIH HHS
Reg. No./Substance:
0/Biocompatible Materials; 0/Membranes, Artificial; 0/Polymers; 0/Sulfones; 25135-51-7/polysulfone P 1700; 9003-39-8/Povidone

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