| Response of ataxia telangiectasia cells to restriction endonuclease induced DNA double-strand breaks: I. Cytogenetic characterization. | |
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MedLine Citation:
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PMID: 8133779 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ataxia telangiectasia (AT) and normal human lymphoblastoid cell lines have been treated with either X-rays or the restriction endonucleases PvuII and BamHI using streptolysin-O poration, and the frequencies of micronuclei or chromosomal aberrations measured. We report that AT cells (AT-PA) are hypersensitive to the restriction endonucleases PvuII and BamHI, inducing DNA double-strand breaks (dsb) with either blunt or cohesive termini, respectively. Our data indicates that AT-PA cells have a dsb processing defect that leads to a higher rate of conversion of dsb into chromosomal aberrations than in normal cells. AT-PA cells showed up to a 5-fold enhanced sensitivity to PvuII over the normal (N-SW) line, a result of an increase in frequencies of chromatid aberrations. Chromosome-type aberrations appeared not to be increased in AT-PA cells over those induced in the normal N-SW line. Particularly striking was the appearance in AT-PA of high frequencies of chromatid aberrations at the 24 h sampling time. BamHI also caused enhanced aberration frequencies in AT-PA cells although the cohesive-ended dsb caused by BamHI still appeared to be less effective in causing chromosomal aberrations than the blunt-ended dsb caused by PvuII in both AT-PA and N-SW, as we have previously reported for Chinese hamster cells. The enhanced effectiveness of cohesive-ended dsb in AT-PA cells over normal cells may be a result of altered processing of dsb by AT-PA cells or may be caused by conversion of some cohesive-ended dsb into blunt-ended dsb by exonuclease digestion before ligation can take place. |
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Authors:
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N Liu; P E Bryant |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Mutagenesis Volume: 8 ISSN: 0267-8357 ISO Abbreviation: Mutagenesis Publication Date: 1993 Nov |
Date Detail:
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Created Date: 1994-04-15 Completed Date: 1994-04-15 Revised Date: 2008-08-19 |
Medline Journal Info:
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Nlm Unique ID: 8707812 Medline TA: Mutagenesis Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 503-10 Citation Subset: IM |
Affiliation:
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School of Biological and Medical Sciences, University of St Andrews, Fife, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Ataxia Telangiectasia
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pathology* Bacterial Proteins Cell Membrane Permeability / drug effects Cells, Cultured Chromosome Aberrations* DNA / drug effects*, radiation effects DNA Damage* Deoxyribonuclease BamHI / pharmacology* Deoxyribonucleases, Type II Site-Specific / pharmacology* Humans Lymphocytes / drug effects, radiation effects, ultrastructure Micronucleus Tests Streptolysins / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Streptolysins; 0/streptolysin O; 9007-49-2/DNA; EC 3.1.21.-/Deoxyribonuclease BamHI; EC 3.1.21.4/CAGCTG-specific type II deoxyribonucleases; EC 3.1.21.4/Deoxyribonucleases, Type II Site-Specific |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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