Document Detail

Response of antioxidant defense system to chromium (VI)-induced cytotoxicity in human diploid cells.
MedLine Citation:
PMID:  19915997     Owner:  NLM     Status:  MEDLINE    
The aim of this study is to establish antioxidant indicators of chromium toxicity in fetal human lung fibroblasts (HLF). The results obtained corroborate and develop our earlier observation of low-dose and long-term action of Cr(VI) on human cells in culture. In the case of a nontoxic chromium dose, temporary oxidative stress is overcome by increased activity of the antioxidant system with correlation to cell cycle re-entry. The toxic concentrations misbalance the cell antioxidant defense systems and cause irreversible growth arrest and massive cell death by apoptosis. Sub-toxicity is defined as toxicity stretched in time. The activity of GPx (glutathione peroxidase) is proposed as a biomarker of oxidative stress caused by Cr(VI), and the GR (glutathione reductase) inhibition is considered as a marker of the toxicity developed under the complex Cr(VI) action. In HLF cells the glutathione dependent defense system is the first system destroyed in response to toxic chromium action. Only the balance between SOD (superoxide dismutase) and H(2)O(2) degrading enzymes (catalase and GPx), should play an important role in the fate of a cell, not individual enzymes.
Nino Asatiani; Marina Abuladze; Tamar Kartvelishvili; Nina Kulikova; Lali Asanishvili; Hoi-Ying Holman; Nelly Sapojnikova
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-15
Journal Detail:
Title:  Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine     Volume:  23     ISSN:  1572-8773     ISO Abbreviation:  Biometals     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-21     Completed Date:  2010-04-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9208478     Medline TA:  Biometals     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  161-72     Citation Subset:  IM    
Andronikashvili Institute of Physics, 0177 Tbilisi, Georgia.
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MeSH Terms
Antioxidants / metabolism*
Apoptosis / drug effects
Catalase / metabolism
Cell Cycle / drug effects
Cell Proliferation / drug effects
Cells, Cultured
Chromium / toxicity*
Dose-Response Relationship, Drug
Drug Evaluation, Preclinical
Fibroblasts / drug effects*,  metabolism
Glutathione Reductase / metabolism
Oxidative Stress / drug effects
Reactive Oxygen Species / metabolism
Structure-Activity Relationship
Superoxide Dismutase / metabolism
Reg. No./Substance:
0/Antioxidants; 0/Reactive Oxygen Species; 18540-29-9/chromium hexavalent ion; 7440-47-3/Chromium; EC; EC Dismutase; EC Reductase

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