| Response of antioxidant defense system to chromium (VI)-induced cytotoxicity in human diploid cells. | |
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MedLine Citation:
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PMID: 19915997 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study is to establish antioxidant indicators of chromium toxicity in fetal human lung fibroblasts (HLF). The results obtained corroborate and develop our earlier observation of low-dose and long-term action of Cr(VI) on human cells in culture. In the case of a nontoxic chromium dose, temporary oxidative stress is overcome by increased activity of the antioxidant system with correlation to cell cycle re-entry. The toxic concentrations misbalance the cell antioxidant defense systems and cause irreversible growth arrest and massive cell death by apoptosis. Sub-toxicity is defined as toxicity stretched in time. The activity of GPx (glutathione peroxidase) is proposed as a biomarker of oxidative stress caused by Cr(VI), and the GR (glutathione reductase) inhibition is considered as a marker of the toxicity developed under the complex Cr(VI) action. In HLF cells the glutathione dependent defense system is the first system destroyed in response to toxic chromium action. Only the balance between SOD (superoxide dismutase) and H(2)O(2) degrading enzymes (catalase and GPx), should play an important role in the fate of a cell, not individual enzymes. |
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Authors:
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Nino Asatiani; Marina Abuladze; Tamar Kartvelishvili; Nina Kulikova; Lali Asanishvili; Hoi-Ying Holman; Nelly Sapojnikova |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-15 |
Journal Detail:
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Title: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine Volume: 23 ISSN: 1572-8773 ISO Abbreviation: Biometals Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-01-21 Completed Date: 2010-04-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9208478 Medline TA: Biometals Country: Netherlands |
Other Details:
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Languages: eng Pagination: 161-72 Citation Subset: IM |
Affiliation:
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Andronikashvili Institute of Physics, 0177 Tbilisi, Georgia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antioxidants
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metabolism* Apoptosis / drug effects Catalase / metabolism Cell Cycle / drug effects Cell Proliferation / drug effects Cells, Cultured Chromium / toxicity* Diploidy* Dose-Response Relationship, Drug Drug Evaluation, Preclinical Fibroblasts / drug effects*, metabolism Glutathione Reductase / metabolism Humans Oxidative Stress / drug effects Reactive Oxygen Species / metabolism Structure-Activity Relationship Superoxide Dismutase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Reactive Oxygen Species; 18540-29-9/chromium hexavalent ion; 7440-47-3/Chromium; EC 1.11.1.6/Catalase; EC 1.15.1.1/Superoxide Dismutase; EC 1.8.1.7/Glutathione Reductase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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