Document Detail

Response of the mitochondrial proteome of rat renal proximal convoluted tubules to chronic metabolic acidosis.
MedLine Citation:
PMID:  23136003     Owner:  NLM     Status:  MEDLINE    
Metabolic acidosis is a common clinical condition that is caused by a decrease in blood pH and bicarbonate concentration. Increased extraction and mitochondrial catabolism of plasma glutamine within the renal proximal convoluted tubule generates ammonium and bicarbonate ions that facilitate the excretion of acid and partially restore acid-base balance. Previous studies identified only a few mitochondrial proteins, including two key enzymes of glutamine metabolism, which are increased during chronic acidosis. A workflow was developed to characterize the mitochondrial proteome of the proximal convoluted tubule. Based upon the increase in specific activity of cytochrome c oxidase, the isolated mitochondria were enriched eightfold. Two-dimensional liquid chromatography coupled with mass spectrometry was utilized to compare mitochondrial-enriched samples from control and chronic acidotic rats. Proteomic analysis identified 901 proteins in the control and acidotic samples. Further analysis identified 37 peptides that contain an N-ε-acetyl-lysine; of these, 22 are novel sites. Spectral counting analysis revealed 33 proteins that are significantly altered in abundance in response to chronic metabolic acidosis. Western blot analysis was performed to validate the calculated changes in abundance. Thus the current study represents the first comprehensive analysis of the mitochondrial proteome of the rat renal proximal convoluted tubule and its response to metabolic acidosis.
Dana M Freund; Jessica E Prenni; Norman P Curthoys
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-11-07
Journal Detail:
Title:  American journal of physiology. Renal physiology     Volume:  304     ISSN:  1522-1466     ISO Abbreviation:  Am. J. Physiol. Renal Physiol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-16     Completed Date:  2013-03-06     Revised Date:  2014-01-23    
Medline Journal Info:
Nlm Unique ID:  100901990     Medline TA:  Am J Physiol Renal Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  F145-55     Citation Subset:  IM    
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MeSH Terms
Acidosis / metabolism*
Ammonium Chloride
Blotting, Western
Chronic Disease
Electron Transport Complex IV / metabolism
Gene Expression Regulation / physiology
Kidney Tubules, Proximal / cytology*,  pathology,  physiology*
Lysine / metabolism
Mitochondria / metabolism*
Peroxisomes / enzymology
Proteome / metabolism*
Rats, Sprague-Dawley
Grant Support
Reg. No./Substance:
0/Proteome; 01Q9PC255D/Ammonium Chloride; EC Transport Complex IV; K3Z4F929H6/Lysine

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