| Response of Halomonas campisalis to saline stress: changes in growth kinetics, compatible solute production and membrane phospholipid fatty acid composition. | |
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MedLine Citation:
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PMID: 17651393 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The haloalkaliphile Halomonas campisalis, isolated near Soap Lake, Washington, was grown under both aerobic and denitrifying conditions from 0 to 260 g L(-1) NaCl, with optimal growth occurring at 20 and 30 g L(-1) NaCl, respectively. Halomonas campisalis was observed to produce high concentrations of compatible solutes, most notably ectoine (up to 500 mM within the cytoplasm), but hydroxyectoine and glycine betaine were also detected. The types and amounts of compatible solutes produced depended on salinity and specific growth rate, as well as on the terminal electron acceptor available (O(2) or NO(3) (-)). A decrease in ectoine production was observed with NO(3) (-) as compared with O(2) as the terminal electron acceptor. In addition, changes in the phospholipid fatty acid composition were measured with changing salinity. An increase in trans fatty acids was observed in the absence of salinity, and may be a response to membrane instability. Cyclic fatty acids were also observed to increase, both in the absence of salinity, and at very high salinities, indicating cell stress at these conditions. |
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Authors:
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John E Aston; Brent M Peyton |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2007-07-25 |
Journal Detail:
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Title: FEMS microbiology letters Volume: 274 ISSN: 0378-1097 ISO Abbreviation: FEMS Microbiol. Lett. Publication Date: 2007 Sep |
Date Detail:
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Created Date: 2007-08-16 Completed Date: 2008-07-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: England |
Other Details:
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Languages: eng Pagination: 196-203 Citation Subset: IM |
Affiliation:
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Department of Chemical and Biological Engineering, Montana State University, Bozeman, MT, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids, Diamino
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metabolism Cell Membrane / chemistry, drug effects*, physiology Fatty Acids / biosynthesis Halomonas / genetics, growth & development*, physiology* Kinetics Osmosis / physiology Phospholipids / biosynthesis Sodium Chloride / pharmacology* Solutions / metabolism Solvents |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids, Diamino; 0/Fatty Acids; 0/Phospholipids; 0/Solutions; 0/Solvents; 7647-14-5/Sodium Chloride; 96702-03-3/ectoine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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