Document Detail


Respirometry: anhydrous drierite equilibrates with carbon dioxide and increases washout times.
MedLine Citation:
PMID:  16927244     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Respirometry is a standard technique in comparative physiology laboratories, with measurements of rates of O(2) consumption and CO(2) production available for hundreds of species. A common recommendation when carrying out respirometry is that water vapor be removed from the air stream before entering the analyzer. Often, this is accomplished with the use of chemical desiccants, such as Drierite. However, here we show that Drierite has an affinity for CO(2) when new and completely anhydrous, and therefore it has an adverse effect on the washout characteristics of this gas. Exposing the Drierite to room air reduces this CO(2) affinity, and a 2-min exposure at 20 degrees Celsius and 50% relative humidity is sufficient to reduce the 99% washout time by almost a factor of 4, at the cost of only a 5% reduction in water vapor capacity. When Drierite is exhausted and then recharged according to the manufacturer's instructions, the CO(2) affinity is further reduced, and washout times are less than 60% greater than when no desiccant is used.
Authors:
Craig R White; Steven J Portugal; Graham R Martin; Patrick J Butler
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-08-16
Journal Detail:
Title:  Physiological and biochemical zoology : PBZ     Volume:  79     ISSN:  1522-2152     ISO Abbreviation:  Physiol. Biochem. Zool.     Publication Date:    2006 Sep-Oct
Date Detail:
Created Date:  2006-08-23     Completed Date:  2006-11-28     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100883369     Medline TA:  Physiol Biochem Zool     Country:  United States    
Other Details:
Languages:  eng     Pagination:  977-80     Citation Subset:  IM    
Affiliation:
Centre for Ornithology, School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom. c.r.white@bham.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Calcium Sulfate / chemistry*
Carbon Dioxide / chemistry*
Physiology, Comparative / methods
Respiratory Physiological Phenomena
Water / chemistry
Chemical
Reg. No./Substance:
124-38-9/Carbon Dioxide; 7732-18-5/Water; 7778-18-9/Calcium Sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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