Document Detail

Respiratory syncytial virus infection of human airway epithelial cells is polarized, specific to ciliated cells, and without obvious cytopathology.
MedLine Citation:
PMID:  11991994     Owner:  NLM     Status:  MEDLINE    
Gene therapy for cystic fibrosis (CF) lung disease requires efficient gene transfer to airway epithelial cells after intralumenal delivery. Most gene transfer vectors so far tested have not provided the efficiency required. Although human respiratory syncytial virus (RSV), a common respiratory virus, is known to infect the respiratory epithelium, the mechanism of infection and the epithelial cell type targeted by RSV have not been determined. We have utilized human primary airway epithelial cell cultures that generate a well-differentiated pseudostratified mucociliary epithelium to investigate whether RSV infects airway epithelium via the lumenal (apical) surface. A recombinant RSV expressing green fluorescent protein (rgRSV) infected epithelial cell cultures with high gene transfer efficiency when applied to the apical surface but not after basolateral inoculation. Analyses of the cell types infected by RSV revealed that lumenal columnar cells, specifically ciliated epithelial cells, were targeted by RSV and that cultures became susceptible to infection as they differentiated into a ciliated phenotype. In addition to infection of ciliated cells via the apical membrane, RSV was shed exclusively from the apical surface and spread to neighboring ciliated cells by the motion of the cilial beat. Gross histological examination of cultures infected with RSV revealed no evidence of obvious cytopathology, suggesting that RSV infection in the absence of an immune response can be tolerated for >3 months. Therefore, rgRSV efficiently transduced the airway epithelium via the lumenal surface and specifically targeted ciliated airway epithelial cells. Since rgRSV appears to breach the lumenal barriers encountered by other gene transfer vectors in the airway, this virus may be a good candidate for the development of a gene transfer vector for CF lung disease.
Liqun Zhang; Mark E Peeples; Richard C Boucher; Peter L Collins; Raymond J Pickles
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  76     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-05-06     Completed Date:  2002-06-10     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5654-66     Citation Subset:  IM    
Cystic Fibrosis/Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, North Carolina 27599-7248, USA.
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MeSH Terms
Antibodies, Viral / immunology
Antiviral Agents / pharmacology
Cell Polarity
Cells, Cultured
Epithelial Cells / metabolism,  pathology,  virology
Genes, Reporter
Green Fluorescent Proteins
Luminescent Proteins
Neutralization Tests
Respiratory Mucosa / cytology
Respiratory Syncytial Virus, Human / physiology*
Ribavirin / pharmacology
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Antibodies, Viral; 0/Antiviral Agents; 0/Luminescent Proteins; 147336-22-9/Green Fluorescent Proteins; 36791-04-5/Ribavirin

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