| Respiratory syncytial virus F and G proteins induce interleukin 1alpha, CC, and CXC chemokine responses by normal human bronchoepithelial cells. | |
| | |
MedLine Citation:
|
PMID: 20205592 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Human respiratory syncytial virus (RSV) is a ubiquitous respiratory virus that causes serious lower respiratory tract disease in infants and young children worldwide. Studies have shown that RSV infection modulates chemokine expression patterns, suggesting that particular cytokine expression profiles may be indicators of disease severity. In this study, we show that RSV F or G protein treatment of fully differentiated primary normal human bronchial epithelial cells induces apical and basolateral secretion of interleukin 8 (IL-8), interferon-inducible protein 10 (IP-10), monocyte chemotactic protein 1 (MCP-1), and RANTES (regulated on activation, normal T cell expressed and secreted). Purified RSV G (attachment) protein was shown to stimulate the secretion of interleukin 1alpha and RANTES, whereas purified F (fusion) protein elicited the production of IL-8, IP-10, and RANTES. Studies of ultraviolet-inactivated RSV showed that treatment of normal human bronchial epithelial cells induces apical IL-8, IP-10, and MCP-1 secretion independent of infection, suggesting that RSV proteins alone modify the chemokine response pattern, which may affect the early immune response before infection. |
| | |
Authors:
|
Christine M Oshansky; James P Barber; Jackelyn Crabtree; Ralph A Tripp |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
|
Title: The Journal of infectious diseases Volume: 201 ISSN: 1537-6613 ISO Abbreviation: J. Infect. Dis. Publication Date: 2010 Apr |
Date Detail:
|
Created Date: 2010-03-15 Completed Date: 2010-04-08 Revised Date: 2012-01-11 |
Medline Journal Info:
|
Nlm Unique ID: 0413675 Medline TA: J Infect Dis Country: United States |
Other Details:
|
Languages: eng Pagination: 1201-7 Citation Subset: AIM; IM |
Affiliation:
|
Department of Infectious Diseases, College of Veterinary Medicine, Center for Disease Intervention, University of Georgia, Athens, Georgia 30602, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adolescent Bronchi / immunology, virology* Cells, Cultured Chemokine CXCL10 / biosynthesis Chemokines, CC / biosynthesis* Chemokines, CXC / biosynthesis* Epithelium / immunology, virology Gene Expression Regulation, Viral / physiology Humans Interleukin-1alpha / biosynthesis* Interleukin-8 / biosynthesis Male Respiratory Syncytial Virus Infections / immunology Respiratory Syncytial Viruses / immunology Viral Fusion Proteins / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
|
R01 AI069275-03/AI/NIAID NIH HHS; R01 AI088744-03/AI/NIAID NIH HHS; R01-AI069275-03/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Chemokine CXCL10; 0/Chemokines, CC; 0/Chemokines, CXC; 0/F protein, human respiratory syncytial virus; 0/G glycoprotein, Respiratory syncytial virus; 0/Interleukin-1alpha; 0/Interleukin-8; 0/Viral Fusion Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Host determinants of HIV-1 control in African Americans.
Next Document: Role of nitric oxide in hyperpnea-induced bronchoconstriction and airway microvascular permeability ...