|Respiration and protein synthesis in Escherichia coli membrane-envelope fragments. IV. Chemical and cytological characterization and biosynthetic capabilities of fragments obtained by mild procedures.|
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|PMID: 4335249 Owner: NLM Status: MEDLINE|
|Membrane-envelope fragments have been isolated from Escherichia coli by comparatively mild techniques. The use of DNAase, RNAase, detergents, sonication, lysozyme, and ethylenediaminetetraacetate were avoided in the belief that rather delicate, but metabolically important, associations may exist between the plasma membrane and various cytoplasmic components. The membrane-envelope fragments have been characterized in terms of their content of major chemical components as well as their electron microscope appearance. Fractions containing membrane-envelope fragments were found to possess appreciable DNA- and protein-synthesizing activities. The fragments were rich in membrane content as determined by reduced nicotinamide adenine dinucleotide (NADH) oxidase activity and deficient in soluble components as measured by NADH dehydrogenase activity. The particulate fraction obtained between 20,000 g and 105,000 g and usually considered a ribosomal fraction was rich in membrane content and had a relatively high capacity for DNA synthesis. Envelope fragments sedimenting at 20,000 g attained very high levels of incorporation of amino acids into protein.|
|R Scharff; R W Hendler; N Nanninga; A H Burgess|
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|Type: Journal Article|
|Title: The Journal of cell biology Volume: 53 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1972 Apr|
|Created Date: 1972-05-31 Completed Date: 1972-05-31 Revised Date: 2010-09-10|
Medline Journal Info:
|Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES|
|Languages: eng Pagination: 1-23 Citation Subset: IM|
|APA/MLA Format Download EndNote Download BibTex|
Amino Acids / metabolism
Bacterial Proteins / biosynthesis*
Cell Membrane / enzymology, metabolism*
Centrifugation, Density Gradient
DNA, Bacterial / biosynthesis*, metabolism
Escherichia coli / cytology, metabolism*
Keto Acids / metabolism
Lipopolysaccharides / metabolism
Oxidoreductases / metabolism
Pimelic Acids / metabolism
Polysaccharides, Bacterial / metabolism
RNA, Bacterial / metabolism
Spheroplasts / metabolism
Thymine Nucleotides / metabolism
|0/Amino Acids; 0/Bacterial Proteins; 0/Carbon Isotopes; 0/DNA, Bacterial; 0/Keto Acids; 0/Lipopolysaccharides; 0/Pimelic Acids; 0/Polysaccharides, Bacterial; 0/RNA, Bacterial; 0/Thymine Nucleotides; 10028-17-8/Tritium; 53-84-9/NAD; EC 1.-/Oxidoreductases|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Journal ID (nlm-ta): J Cell Biol
Publisher: The Rockefeller University Press
Copyright © 1972 by The Rockefeller University Press
Received Day: 2 Month: 2 Year: 1971
Revision Received Day: 4 Month: 11 Year: 1971
Print publication date: Day: 1 Month: 4 Year: 1972
Volume: 53 Issue: 1
First Page: 1 Last Page: 23
PubMed Id: 4335249
|RESPIRATION AND PROTEIN SYNTHESIS IN ESCHERICHIA COLI MEMBRANE-ENVELOPE FRAGMENTS : IV. Chemical and Cytological Characterization and Biosynthetic Capabilities of Fragments Obtained by Mild Procedures|
|R. W. Hendler|
|A. H. Burgess|
|From the Section on Cellular Physiology, Laboratory of Biochemistry, National Heart and Lung Institute, National Institutes of Health, Bethesda, Maryland 20014, and the Laboratory for Electron Microscopy, University of Amsterdam, Netherlands
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