| Respiration in Mycobacterium leprae. | |
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MedLine Citation:
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PMID: 3003214 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fairly pure leprosy bacilli were easily collected from nude mouse foot pad lepromas by the Ficoll density gradient centrifugation and alkali treatment methods. The yield of bacilli available for biochemical study was 42.6%. The density of Mycobacterium leprae was very heterogeneous. The percent of solid bacilli in the light bacilli fraction was 23%; that in the heavy bacilli fraction was 40%. The endogenous respiration activity in the heavy bacilli was greater than that in light bacilli. The average coefficient of respiration in M. leprae was 1 microliter O2/mg X hr. In the whole cells of M. leprae, a cytochrome b1 absorption peak and its Soret peak were detected at wavelengths of 560 nm and 426 nm, respectively. However, a cytochrome a2-like peak (which was observed in M. lepraemurium), and a cyt c and cyt a were not detected. Catalase activity was not found in whole cells, the cell-free extract, or particle fractions of M. leprae. Any catalase activity associated with M. leprae suspensions is a tissue contaminant. NAD-peroxidase activity was also not detected in the cell-free extract of the leprosy bacillus. These results would indicate that leprosy bacilli cannot degrade hydrogen peroxide. |
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Authors:
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T Mori; Y Miyata; K Kohsaka; M Makino |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association Volume: 53 ISSN: 0148-916X ISO Abbreviation: Int. J. Lepr. Other Mycobact. Dis. Publication Date: 1985 Dec |
Date Detail:
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Created Date: 1986-03-26 Completed Date: 1986-03-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8505819 Medline TA: Int J Lepr Other Mycobact Dis Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 600-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Catalase / metabolism Centrifugation, Density Gradient Cytochromes / metabolism Leprosy / microbiology Mice Mycobacterium leprae / enzymology*, isolation & purification, metabolism Nucleotidases / metabolism Oxidoreductases / metabolism Oxygen Consumption Peroxidases / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Cytochromes; EC 1.-/Oxidoreductases; EC 1.11.1.-/Peroxidases; EC 1.11.1.1/NAD+ peroxidase; EC 1.11.1.6/Catalase; EC 3.1.3.-/Nucleotidases; EC 3.1.3.-/adenosine monophosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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