Document Detail

Respiration in Mycobacterium leprae.
MedLine Citation:
PMID:  3003214     Owner:  NLM     Status:  MEDLINE    
Fairly pure leprosy bacilli were easily collected from nude mouse foot pad lepromas by the Ficoll density gradient centrifugation and alkali treatment methods. The yield of bacilli available for biochemical study was 42.6%. The density of Mycobacterium leprae was very heterogeneous. The percent of solid bacilli in the light bacilli fraction was 23%; that in the heavy bacilli fraction was 40%. The endogenous respiration activity in the heavy bacilli was greater than that in light bacilli. The average coefficient of respiration in M. leprae was 1 microliter O2/mg X hr. In the whole cells of M. leprae, a cytochrome b1 absorption peak and its Soret peak were detected at wavelengths of 560 nm and 426 nm, respectively. However, a cytochrome a2-like peak (which was observed in M. lepraemurium), and a cyt c and cyt a were not detected. Catalase activity was not found in whole cells, the cell-free extract, or particle fractions of M. leprae. Any catalase activity associated with M. leprae suspensions is a tissue contaminant. NAD-peroxidase activity was also not detected in the cell-free extract of the leprosy bacillus. These results would indicate that leprosy bacilli cannot degrade hydrogen peroxide.
T Mori; Y Miyata; K Kohsaka; M Makino
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association     Volume:  53     ISSN:  0148-916X     ISO Abbreviation:  Int. J. Lepr. Other Mycobact. Dis.     Publication Date:  1985 Dec 
Date Detail:
Created Date:  1986-03-26     Completed Date:  1986-03-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8505819     Medline TA:  Int J Lepr Other Mycobact Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  600-9     Citation Subset:  IM    
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MeSH Terms
Catalase / metabolism
Centrifugation, Density Gradient
Cytochromes / metabolism
Leprosy / microbiology
Mycobacterium leprae / enzymology*,  isolation & purification,  metabolism
Nucleotidases / metabolism
Oxidoreductases / metabolism
Oxygen Consumption
Peroxidases / metabolism
Reg. No./Substance:
0/Cytochromes; EC 1.-/Oxidoreductases; EC 1.11.1.-/Peroxidases; EC peroxidase; EC; EC 3.1.3.-/Nucleotidases; EC 3.1.3.-/adenosine monophosphatase

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