Document Detail

Respective role of uraemic toxins and myeloperoxidase in the uraemic state.
MedLine Citation:
PMID:  16476719     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: In haemodialysis (HD) patients, advanced oxidation protein products (AOPP) were previously ascribed to oxidized plasma proteins, resulting mainly from increased myeloperoxidase (MPO) activity. The aim of the present study was to assess the mechanisms leading to the generation of AOPP during the course of chronic kidney disease including end-stage renal disease, with particular focus on AOPP and MPO characterization in the plasma at decreasing levels of kidney function. METHODS: Phagocyte activation was evaluated by whole blood NADPH oxidase and MPO activities. In plasma, MPO protein concentration was quantified by ELISA and catalytic activity assayed by the spectrophotometric detection of phenol and 4-aminoantipyrine (AAP) co-oxidation in the presence of hydrogen peroxide (H(2)O(2)). RESULTS: In HD patients, plasma AOPP concentration was linked to neutrophil oxidative activity. Such an association was not found in control subjects or predialysis patients, suggesting that in the latter, AOPP generation did not mainly result from MPO released by activated neutrophils. Similarly, plasma AOPP correlated with plasma MPO protein concentration in HD patients, but not in control subjects or predialysis patients, suggesting that in the latter AOPP did not predominantly result from MPO activity. This interpretation was supported by the observation of a greater degree of co-oxidation of phenol and AAP in the absence of H(2)O(2) in predialysis patients than in HD patients or control subjects. The contribution of MPO dramatically differed between predialysis and HD patients (2+/-5 vs 46+/-6%; P<0.001). CONCLUSION: Our observations suggest that AOPP generation in predialysis patients mainly results from MPO-independent oxidation mechanisms.
Chantal Capeillère-Blandin; Valérie Gausson; Anh Thu Nguyen; Béatrice Descamps-Latscha; Tilman Drüeke; Véronique Witko-Sarsat
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-02-13
Journal Detail:
Title:  Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association     Volume:  21     ISSN:  0931-0509     ISO Abbreviation:  Nephrol. Dial. Transplant.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-05-22     Completed Date:  2006-11-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8706402     Medline TA:  Nephrol Dial Transplant     Country:  England    
Other Details:
Languages:  eng     Pagination:  1555-63     Citation Subset:  IM    
Université Paris 5, CNRS UMR 8601, 45 rue des Saints Pères, 75270 Paris Cedex 06, France.
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MeSH Terms
Case-Control Studies
Kidney Failure, Chronic / enzymology,  urine*
Middle Aged
Peroxidase / metabolism,  physiology*
Phagocytes / metabolism
Phenol / metabolism
Proteins / metabolism
Renal Dialysis
Toxins, Biological / urine*
Uremia / enzymology,  etiology*
Reg. No./Substance:
0/Proteins; 0/Toxins, Biological; 108-95-2/Phenol; EC

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