Document Detail


Respective role of the 42- and 51-kDa components of the Bacillus sphaericus toxin overexpressed in Bacillus thuringiensis.
MedLine Citation:
PMID:  8384140     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The 42- and 51-kDa protein genes of Bacillus sphaericus 1593 have been subcloned independently downstream from the cytA gene promoter of Bacillus thuringiensis serovar israelensis and introduced into a non-mosquitocidal strain of Bacillus thuringiensis. Consequently, each protein was overproduced and accumulated as inclusion bodies which were purified. For the first time, the 42-kDa protein inclusions alone were found to be toxic to Culex pipiens larvae (LC50 at 48 h 300 ng ml-1); in contrast, the 51-kDa protein inclusions were not. Moreover, a synergistic effect between these two components was observed.
Authors:
L Nicolas; C Nielsen-Leroux; J F Charles; A Delécluse
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  106     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  1993 Feb 
Date Detail:
Created Date:  1993-04-22     Completed Date:  1993-04-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  275-80     Citation Subset:  IM    
Affiliation:
Unité des Bactéries Entomopathogènes, Institut Pasteur, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacillus / genetics*
Bacillus thuringiensis / genetics,  ultrastructure
Bacterial Toxins / chemistry,  genetics,  toxicity*
Cloning, Molecular
Culex / drug effects
Gene Expression
Genes, Bacterial
Inclusion Bodies / ultrastructure
Larva / drug effects
Microscopy, Electron
Molecular Weight
Plasmids
Restriction Mapping
Chemical
Reg. No./Substance:
0/Bacterial Toxins; 0/binB protein, Bacillus sphaericus

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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