Document Detail


Respective hydrolysis and esterification of esterified and free plant stanols occur rapidly in human intestine after their duodenal infusion in triacyl- or diacylglycerol.
MedLine Citation:
PMID:  17551763     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Esterification of dietary phytosterols and glycerols may affect intestinal absorption of cholesterol and non-cholesterol sterols. We infused plant stanol esters in triacylglycerol (TAG) (F1) and diacylglycerol (DG) (F2) oils, and free plant stanols in F1 and F2 (F3) to the duodenum of healthy human subjects and sampled the contents from the proximal jejunum (PJ). Free and ester sterols were analysed from the infusates, and intestinal contents before and after ultracentrifuge separation of oil, micelle and sediment phases. During the 60-cm intestinal passage, over 40% of plant stanol esters were hydrolysed (P < 0.05) but around 30% of the infused free plant stanols (P < 0.05) and up to 40% of cholesterol (P < 0.05) were esterified in PJ after infusions. TAG in F1 favoured accumulation of plant stanol esters in the oil phase of the PJ aspirates as compared with respective values of F2 and F3 (P < 0.05 for both). About one third of free plant stanols of F3 had been esterified (P < 0.05) and 17% precipitated mainly in free form in the PJ aspirates (P < 0.05 compared with F1 and F2). In conclusion, DG- and TAG-oils had no profound superiority over each other as intestinal carriers regarding hydrolysis/esterification of administered plant stanol esters and cholesterol and their partition in oil, micellar and sediment phases in the PJ. The unesterified plant stanols experienced partial esterification and sedimentation during their intestinal passage, which might influence their biochemical properties in that segment of the gut where cholesterol is absorbed.
Authors:
Markku J Nissinen; Matti Vuoristo; Helena Gylling; Tatu A Miettinen
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-06
Journal Detail:
Title:  Lipids     Volume:  42     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-02     Completed Date:  2008-01-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  United States    
Other Details:
Languages:  eng     Pagination:  603-12     Citation Subset:  IM    
Affiliation:
Department of Medicine, Division of Gastroenterology, University of Helsinki, Helsinki 00029, Finland. markku.nissinen@dlc.fi
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MeSH Terms
Descriptor/Qualifier:
Adult
Diglycerides / metabolism*
Duodenum / metabolism*
Esterification
Female
Humans
Hydrolysis
Intestinal Absorption / physiology*
Intestine, Small / metabolism*
Jejunum / metabolism
Male
Phytosterols / metabolism*
Sitosterols / metabolism*
Triglycerides / metabolism*
Chemical
Reg. No./Substance:
0/1,2-diacylglycerol; 0/Diglycerides; 0/Phytosterols; 0/Sitosterols; 0/Triglycerides; 0/plant stanol ester

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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