Document Detail


Resorcinol as a Spectrofluorometric Probe for the Hypochlorous Acid Scavenging Activity Assay of Biological Samples.
MedLine Citation:
PMID:  23046424     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A novel spectrofluorimetric method was developed and validated for hypochlorous acid (HOCl) scavenging activity estimation using resorcinol, a highly sensitive and chemically stable fluorogenic probe. This assay is based on the chlorination of resorcinol to its non-fluorescent products in the presence of HOCl. HOCl reacts with both resorcinol and HOCl-scavengers incubated in solution for 10 min where scavengers compete with resorcinol for the HOCl. Thus, the relative increase in fluorescence intensity of intact resorcinol is proportional to the antioxidative activity of HOCl scavengers. Using this reaction, a kinetic approach was adopted to assess the HOCl scavenging activity of amino acids, vitamins, plasma- and thiol- antioxidants. This assay, applicable to small molecule antioxidants and tissue homogenates, proved to be efficient for thiol-type antioxidants for which the widely used TNB test is not accurately responsive. Thus, conventional problems of the TNB assay arising from reactivity of thiol-type scavengers to produce extra TNB by direct reduction of DTNB were overcome. Moreover, unlike enzymatic (e.g., elastase) assays, there is no confusion as to whether the putative scavenger actually reacts with HOCl or inhibits the enzyme.
Authors:
Mustafa Ozyürek; Burcu Bekdeşer; Kubilay Güçlü; Reşat Apak
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-9
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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