Document Detail

Resorcinol as a Spectrofluorometric Probe for the Hypochlorous Acid Scavenging Activity Assay of Biological Samples.
MedLine Citation:
PMID:  23046424     Owner:  NLM     Status:  Publisher    
A novel spectrofluorimetric method was developed and validated for hypochlorous acid (HOCl) scavenging activity estimation using resorcinol, a highly sensitive and chemically stable fluorogenic probe. This assay is based on the chlorination of resorcinol to its non-fluorescent products in the presence of HOCl. HOCl reacts with both resorcinol and HOCl-scavengers incubated in solution for 10 min where scavengers compete with resorcinol for the HOCl. Thus, the relative increase in fluorescence intensity of intact resorcinol is proportional to the antioxidative activity of HOCl scavengers. Using this reaction, a kinetic approach was adopted to assess the HOCl scavenging activity of amino acids, vitamins, plasma- and thiol- antioxidants. This assay, applicable to small molecule antioxidants and tissue homogenates, proved to be efficient for thiol-type antioxidants for which the widely used TNB test is not accurately responsive. Thus, conventional problems of the TNB assay arising from reactivity of thiol-type scavengers to produce extra TNB by direct reduction of DTNB were overcome. Moreover, unlike enzymatic (e.g., elastase) assays, there is no confusion as to whether the putative scavenger actually reacts with HOCl or inhibits the enzyme.
Mustafa Ozyürek; Burcu Bekdeşer; Kubilay Güçlü; Reşat Apak
Related Documents :
24518334 - Chemical and enzymatic synthesis of a library of 2-phenethyl esters and their sensory a...
24258144 - Industrial production of l-ascorbic acid (vitamin c) and d-isoascorbic acid.
24731374 - A novel method for predicting antioxidant activity based on amino acid structure.
24620834 - Analysis of flavonoids and hydroxycinnamic acid derivatives in rapeseeds (brassica napu...
15348094 - Incorporation of amino acids within the surface reactive layers of bioactive glass in v...
11575804 - Accurate molecular mass determination of mycolic acids by maldi-tof mass spectrometry.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-9
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Genotype-specific SNP map based on whole chromosome 3B sequence information from wheat cultivars Ari...
Next Document:  Electrically-Active Screw Dislocations in Helical ZnO and Si Nanowires and Nanotubes.