Document Detail

Resonance light scattering imaging detection of proteins with alpha,beta,gamma,delta-tetrakis(p-sulfophenyl)porphyrin.
MedLine Citation:
PMID:  14511689     Owner:  NLM     Status:  MEDLINE    
A resonance light scattering (RLS) imaging technique was introduced to measure the light scattering of aggregation species induced by proteins, and thus a method of detecting proteins in the range of picograms was proposed. In acidic medium, J-aggregation of alpha,beta,gamma,delta-tetrakis(p-sulfophenyl)porphyrin (TPPS(4)) in the presence of proteins occurs, resulting in strong RLS signals characterized at 490 nm. Under the excitation of a 488-nm light beam of argon ion laser source, the scattered light of single J-aggregation species could be observed with a common microscope, and the images could be captured with a cooled charge-coupled device camera. Data analysis for the digital images showed that the counts of aggregate species in the detection focus plane are proportional to the concentration of proteins in picograms. When 1.0 x 10(-7)M TPPS(4) was employed, 0.01-210 ng/ml bovine serum albumin and human serum albumin could be detected with limits of detection lower than 10 pg/ml (3 sigma). Three human blood serum samples were satisfactorily detected with relative standard deviations lower than 3.04%.
Cheng Zhi Huang; Ying Liu; Yong Hong Wang; Hong Ping Guo
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical biochemistry     Volume:  321     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-09-26     Completed Date:  2004-06-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  236-43     Citation Subset:  IM    
Laboratory of Supramolecular Chemistry and Biomedical Analysis, College of Chemistry and Chemical Engineering, Southwest Normal University, Chongqing 400715, People's Republic of China.
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MeSH Terms
Blood Proteins / analysis
Image Enhancement
Macromolecular Substances
Microscopy / methods*
Porphyrins / chemistry*
Proteins / analysis*
Scattering, Radiation
Spectrometry, Fluorescence
Reg. No./Substance:
0/Blood Proteins; 0/Macromolecular Substances; 0/Porphyrins; 0/Proteins; 35218-75-8/tetraphenylporphine sulfonate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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