Document Detail


Resolution of sister telomere association is required for progression through mitosis.
MedLine Citation:
PMID:  15064417     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cohesins keep sister chromatids associated from the time of their replication in S phase until the onset of anaphase. In vertebrate cells, two distinct pathways dissociate cohesins, one acts on chromosome arms and the other on centromeres. Here, we describe a third pathway that acts on telomeres. Knockdown of tankyrase 1, a telomeric poly(ADP-ribose) polymerase caused mitotic arrest. Chromosomes aligned normally on the metaphase plate but were unable to segregate. Sister chromatids separated at centromeres and arms but remained associated at telomeres, apparently through proteinaceous bridges. Thus, telomeres may require a unique tankyrase 1-dependent mechanism for sister chromatid resolution before anaphase.
Authors:
Jasmin N Dynek; Susan Smith
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  304     ISSN:  1095-9203     ISO Abbreviation:  Science     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-04-05     Completed Date:  2004-04-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  97-100     Citation Subset:  IM    
Affiliation:
Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA.
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MeSH Terms
Descriptor/Qualifier:
Anaphase*
Chromatids / physiology*
Chromosome Segregation
DNA Replication
Hela Cells
Humans
In Situ Hybridization, Fluorescence
Metaphase
Mitosis*
Phenotype
RNA, Small Interfering / metabolism
Tankyrases / genetics,  metabolism*
Telomere / physiology*
Transfection
Ubiquitin-Protein Ligase Complexes / metabolism
Grant Support
ID/Acronym/Agency:
GM07238-28/GM/NIGMS NIH HHS; R01 CA95099-01/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Small Interfering; EC 2.4.2.30/Tankyrases; EC 2.4.4.30/TNKS protein, human; EC 6.3.2.19/Ubiquitin-Protein Ligase Complexes; EC 6.3.2.19/anaphase-promoting complex
Comments/Corrections
Comment In:
Science. 2004 Apr 2;304(5667):60-2   [PMID:  15064409 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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