Document Detail


Resolution of the major hemorrhagic component of Trimeresurus flavoviridis venom into two parts.
MedLine Citation:
PMID:  518906     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. The major hemorrhagic component (HR1) in the venom of Trimeresurus flavoviridis was purified further by gel filtration on Sephadex G-200, superfine, resulting in its resolution into two parts, 1A and 1B. 1A possessed proteolytic activity towards casein, while 1B was almost free from such activity. Both components were associated with lethal toxicity. 2. The purified preparations of 1A and 1B were homogeneous as judged by several criteria. The molecular weights of the purified principles determined by dodecyl sulfate gel electrophoresis were approximately 60 000. 1A shows anomalous behaviour on ultracentrifugation and gel filtration owing to concentration-dependent polymeric interaction. The purified components were acidic glycoproteins with isoelectric points of 4.4 and they contained neutral sugar, amino sugar and sialic acid altogether amounting to 17-18% on the total weight basis. 3. The two hemorrhagic components were closely related, if not identical, immunologically.
Authors:
T Omori-Satoh; S Sadahiro
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  580     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1979 Oct 
Date Detail:
Created Date:  1980-03-24     Completed Date:  1980-03-24     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  392-404     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Chemical Phenomena
Chemistry
Chemistry, Physical
Chromatography, Gel
Crotalid Venoms / analysis*,  immunology
Electrophoresis, Polyacrylamide Gel
Hemorrhage / chemically induced*
Molecular Weight
Physicochemical Phenomena
Rabbits
Ultracentrifugation
Chemical
Reg. No./Substance:
0/Crotalid Venoms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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