Document Detail

Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst.
MedLine Citation:
PMID:  20412781     Owner:  NLM     Status:  MEDLINE    
Three distinct cell types are present within the 64-cell stage mouse blastocyst. We have investigated cellular development up to this stage using single-cell expression analysis of more than 500 cells. The 48 genes analyzed were selected in part based on a whole-embryo analysis of more than 800 transcription factors. We show that in the morula, blastomeres coexpress transcription factors specific to different lineages, but by the 64-cell stage three cell types can be clearly distinguished according to their quantitative expression profiles. We identify Id2 and Sox2 as the earliest markers of outer and inner cells, respectively. This is followed by an inverse correlation in expression for the receptor-ligand pair Fgfr2/Fgf4 in the early inner cell mass. Position and signaling events appear to precede the maturation of the transcriptional program. These results illustrate the power of single-cell expression analysis to provide insight into developmental mechanisms. The technique should be widely applicable to other biological systems.
Guoji Guo; Mikael Huss; Guo Qing Tong; Chaoyang Wang; Li Li Sun; Neil D Clarke; Paul Robson
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental cell     Volume:  18     ISSN:  1878-1551     ISO Abbreviation:  Dev. Cell     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-23     Completed Date:  2010-05-06     Revised Date:  2010-05-10    
Medline Journal Info:
Nlm Unique ID:  101120028     Medline TA:  Dev Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  675-85     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
Department of Biological Sciences, National University of Singapore, Singapore 117543.
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MeSH Terms
Blastocyst / cytology*
Cell Cycle
Cell Differentiation
Cell Lineage
Developmental Biology
Gene Expression Profiling*
Gene Expression Regulation, Developmental*
Signal Transduction
Transcription, Genetic
Transcriptional Activation
Zygote / cytology*
Comment In:
Cell Stem Cell. 2010 May 7;6(5):397-8   [PMID:  20452308 ]

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