Document Detail


Resolution of apolipoprotein B repeat unit position variants on agarose, denaturing, and native polyacrylamide gels.
MedLine Citation:
PMID:  8855402     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Utilizing three different electrophoresis systems for the separation of variable number of tandem repeats (VNTR) alleles of the high variable region 3' from the apolipoprotein B (Apo B) locus, it could be shown that apparent allele subtypes on native polyacrylamide gels comigrated with the regular ladder alleles on agarose and denaturing polyacrylamide gels. Two subtypes, one with a shift towards the cathode and one shifted towards the anode, and the corresponding ladder alleles were sequenced in order to determine the molecular basis for this aberrant electrophoretical mobility. Subtype and ladder alleles displayed the same number of repeat units but differed in the number and order of different types of repeats. Depending on the variation in the region of pure AT repeat units, the potential secondary structures of hairpin loop formation differed for subtypes and ladder alleles, which is thought to explain the shift in electrophoretic mobility on native polyacrylamide gel electrophoresis.
Authors:
M Prinz; C Schmitt; M Staak; H Baum
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Electrophoresis     Volume:  17     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1996-12-19     Completed Date:  1996-12-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  1190-3     Citation Subset:  IM    
Affiliation:
Department of Forensic Biology, Office of Chief Medical Examiner, New York, NY 10016, USA.
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MeSH Terms
Descriptor/Qualifier:
Apolipoproteins B / genetics*
Base Sequence
DNA / chemistry,  isolation & purification*
Electrophoresis, Polyacrylamide Gel / methods*
Humans
Nucleic Acid Conformation
Polymerase Chain Reaction
Protein Denaturation*
Repetitive Sequences, Nucleic Acid
Sepharose*
Chemical
Reg. No./Substance:
0/Apolipoproteins B; 9007-49-2/DNA; 9012-36-6/Sepharose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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