| Resolution of alkenylacylglycerol moieties of natural glycerophospholipids by gas-liquid chromatography on polar capillary columns. | |
| | |
MedLine Citation:
|
PMID: 6467078 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A sensitive and practical procedure has been developed for the determination of the sn-1-alk-1-enyl-2-acylglycerol moieties of natural glycerophospholipids using capillary gas-liquid chromatography with flame ionization detection. The method gives excellent resolution and quantitative estimates for molecular species according to carbon number and degree of unsaturation. The diradylglycerols are released from the glycerophospholipids by hydrolysis with phospholipase C and the alk-1-enylacylglycerols are resolved by thin-layer chromatography. Either the trimethylsilyl or the tertiary-butyldimethylsilyl ethers are then resolved at 250 degrees C by gas-liquid chromatography on a glass capillary column wall-coated with a polar cyanopropylsiloxane polymer using hydrogen as the carrier gas. The method has been applied to the analysis of the alk-1-enylacyl- and diacyl-glycerol moieties of the phosphatidylethanolamines of rat heart and kidney. |
| | |
Authors:
|
J J Myher; A Kuksis |
Related Documents
:
|
1877748 - On-column sample gating for high-speed capillary zone electrophoresis. 12179988 - Fluorescein-based pi markers for capillary isoelectric focusing with laser-induced fluo... 23509898 - Adsorption and separation of co2 on kfi zeolites: effect of cation type and si/al ratio... 2738138 - Host-guest complexation in capillary isotachophoresis. ii. determination of aminophenol... 15517528 - On-line analysis of diesel engine exhaust gases by selected ion flow tube mass spectrom... 19072898 - Liquid-phase microextraction by solidification of floating organic microdrop and gc-ms ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire Volume: 62 ISSN: 0714-7511 ISO Abbreviation: Can. J. Biochem. Cell Biol. Publication Date: 1984 Jun |
Date Detail:
|
Created Date: 1984-10-11 Completed Date: 1984-10-11 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8302763 Medline TA: Can J Biochem Cell Biol Country: CANADA |
Other Details:
|
Languages: eng Pagination: 352-62 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Chromatography, Gas / methods Fatty Acids, Unsaturated / analysis* Gas Chromatography-Mass Spectrometry / methods Glycerides / analysis* Kidney / analysis Myocardium / analysis Phosphatidic Acids / analysis* Phosphatidylethanolamines / analysis Phospholipids / analysis* Rats Type C Phospholipases / diagnostic use |
| Grant Support | |
ID/Acronym/Agency:
|
NIH-NHLI-72-917/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Fatty Acids, Unsaturated; 0/Glycerides; 0/Phosphatidic Acids; 0/Phosphatidylethanolamines; 0/Phospholipids; EC 3.1.4.-/Type C Phospholipases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Endogenous and exogenous factors affecting ribosomal RNA release from rat liver nuclei in a cell-fre...
Next Document: Biosynthetic utilization of photoreactive fatty acids by rat liver microsomes.