| Resistance to scrapie in PrP ARR/ARQ heterozygous sheep is not caused by preferential allelic use. | |
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MedLine Citation:
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PMID: 15166274 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: In sheep, susceptibility to scrapie, which is similar to human prion diseases such as Kuru and variant Creutzfeldt-Jakob disease (vCJD), is determined by prion protein (PrP) gene (Prnp) polymorphisms. Sheep with genotype ARQ/ARQ, denoting polymorphisms at codons 136, 154, and 171, are susceptible, whereas those with genotypes ARR/ARQ and ARR/ARR are resistant, indicating dominance of ARR over the ARQ allele. AIMS: Based on familial CJD E200K, 129V, where preferential use of the 200E allele in EK heterozygous individuals confers resistance, heterozygous ARR/ARQ sheep were used to test the hypothesis that resistance is caused by preferential use of the ARR allele. METHODS: After assessment of equivalent PrP expression across genotypes, allele use was analysed by sequencing reverse transcription polymerase chain reaction derived DNA clones containing the Prnp gene coding sequence. RESULTS: The ARR to ARQ ratio was 1.1 in 133 clones, representing Prnp mRNA from three ARR/ARQ sheep, indicating equal use of both alleles. CONCLUSIONS: Dominance of the resistant associated allele in sheep scrapie involves mechanisms other than the absence of PrP derived from the disease associated ARQ allele. |
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Authors:
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P A Caplazi; K I O'Rourke; T V Baszler |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of clinical pathology Volume: 57 ISSN: 0021-9746 ISO Abbreviation: J. Clin. Pathol. Publication Date: 2004 Jun |
Date Detail:
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Created Date: 2004-05-28 Completed Date: 2004-06-21 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0376601 Medline TA: J Clin Pathol Country: England |
Other Details:
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Languages: eng Pagination: 647-50 Citation Subset: AIM; IM |
Affiliation:
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Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alleles Animals Fluorescent Antibody Technique Genetic Predisposition to Disease Genotype Heterozygote Prions / genetics*, metabolism Scrapie / genetics*, metabolism Sheep |
| Chemical | |
Reg. No./Substance:
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0/Prions |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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